Coomassie Brilliant Blue G is a more potent antagonist of P2 purinergic responses than Reactive Blue 2 (Cibacron Blue 3GA) in rat parotid acinar cells.
Coomassie Brilliant Blue G is a more potent antagonist of P2 purinergic responses than Reactive Blue 2 (Cibacron Blue 3GA) in rat parotid acinar cells.
复制标题
在大鼠腮腺腺泡细胞中，考马斯亮蓝 G 是比活性蓝 2 (Cibacron Blue 3GA) 更有效的 P2 嘌呤能反应拮抗剂。
DOI：
10.1016/0006-291x(89)92741-1
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发表时间：
1989
影响因子：
3.1
通讯作者：
Talamo,BR
中科院分区：
文献类型：
--
作者：
Soltoff,SP;McMillian,MK;Talamo,BR
通过评估亮蓝 G (考马斯亮蓝 G) 和活性蓝 2 (Cibacron Blue 3GA) 对 ATP 刺激的 45Ca2+ 进入和 [Ca2+]i（Fura 2 荧光）升高的影响，检查其阻断细胞外 ATP 对大鼠腮腺腺泡细胞的影响的能力。 ATP (300 μM) 使 Ca2+ 进入速率增加至基础速率的 25 倍以上，并使 [Ca2+]ito 水平升高至基础值的三倍以上。亮蓝G和活性蓝2大大减少了45Ca2+进入腮腺细胞，但亮蓝G的效力（IC50∼0.4 μM）约为活性蓝2的100倍。Fura 2研究表明，这些化合物的抑制浓度不会阻断这些细胞的胆碱能反应，从而证明了染料化合物对嘌呤能受体的选择性。与活性蓝 2 不同，有效浓度的亮蓝 G 不会显着淬灭 Fura 2 荧光。亮蓝 G 的更强效力表明它在识别 P2 型嘌呤能受体方面可能非常有用，特别是在使用荧光探针的研究中。
The ability of Brilliant Blue G (Coomassie Brilliant Blue G) and Reactive Blue 2 (Cibacron Blue 3GA) to block the effects of extracellular ATP on rat parotid acinar cells was examined by evaluating their effects on ATP-stimulated45Ca2+entry and the elevation of [Ca2+]i(Fura 2 fluorescence). ATP (300 μM) increased the rate of Ca2+entry to more than 25-times the basal rate and elevated [Ca2+]ito levels more than three times the basal value. Brilliant Blue G and Reactive Blue 2 greatly reduced the entry of45Ca2+into parotid cells, but the potency of Brilliant Blue G (IC50∼0.4 μM) was about 100-times that of Reactive Blue 2. Fura 2 studies demonstrated that inhibitory concentrations of these compounds did not block the cholinergic response of these cells, thus demonstrating the selectivity of the dye compounds for purinergic receptors. Unlike Reactive Blue 2, effective concentrations of Brilliant Blue G did not substantially quench Fura 2 fluorescence. The greater potency of Brilliant Blue G suggests that it may be very useful in identifying P2-type purinergic receptors, especially in studies which utilize fluorescent probes.