Transcriptional Profiling of the Oral Pathogen Streptococcus mutans in Response to Competence Signaling Peptide XIP.
Transcriptional Profiling of the Oral Pathogen Streptococcus mutans in Response to Competence Signaling Peptide XIP.
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DOI：
10.1128/msystems.00102-16
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发表时间：
2017-01
期刊：
影响因子：
6.4
通讯作者：
Cvitkovitch DG
中科院分区：
文献类型：
--
作者：
Wenderska IB;Latos A;Pruitt B;Palmer S;Spatafora G;Senadheera DB;Cvitkovitch DG
遗传能力为细菌提供了增加遗传多样性或获得赋予生存优势的新特性的机会。在致龋病原体变形链球菌中，DNA转化受感受态刺激肽XIP（ComX诱导肽）调节。本研究利用高通量RNA测序（RNAseq）来更好地了解全球基因表达模式如何响应XIP而变化。总的来说，我们的工作表明，在变形链球菌中，XIP信号转导诱导的反应类似于对氨基酸饥饿的严格反应。我们进一步确定了一个新的热休克反应基因间区域的能力关闭的潜在作用。总之，我们的研究结果提供了进一步的证据，表明多种应激反应机制是通过变异链球菌的遗传能力信号通路联系在一起的。在致龋性变形链球菌中，感受态发育受ComRS信号系统的调节，该系统由ComR调节子和ComS前肽组成，以感受态信号肽XIP（ComX诱导肽）。除了感受态发育之外，XIP信号传导已被证明可调节细胞裂解，并且最近还可调节细菌素的表达，细菌素是细菌用于抑制密切相关物种的小抗微生物肽。我们的研究进一步探索了XIP信号对变形链球菌转录组的影响。RNA测序显示，XIP诱导导致基因表达的整体变化，这与应激反应一致。观察到几种膜结合调节剂的增加，包括参与细菌素生产的HdrRM和BrsRM，以及参与耐酸性和生物膜形成的VicRKX系统。此外，基因表达的全球变化对应于在对氨基酸饥饿的严格响应期间观察到的变化。还观察到对参与糖转运和碳分解代谢物阻遏的基因的影响，包括levQlR和levDEFG操纵子。最后，我们的工作确定了一个新的热休克反应基因间区域，编码一个小RNA，在能力关闭的潜在作用。遗传能力为细菌提供了增加遗传多样性或获得赋予生存优势的新特性的机会。在致龋病原体变形链球菌中，DNA转化受到感受态刺激肽XIP（ComX诱导肽）的调节。本研究利用高通量RNA测序（RNAseq）来更好地了解全球基因表达模式如何响应XIP而变化。总的来说，我们的工作表明，在变形链球菌中，XIP信号转导诱导的反应类似于对氨基酸饥饿的严格反应。我们进一步确定了一个新的热休克反应基因间区域的能力关闭的潜在作用。总之，我们的研究结果提供了进一步的证据，表明多种应激反应机制是通过变异链球菌的遗传能力信号通路联系在一起的。
Genetic competence provides bacteria with an opportunity to increase genetic diversity or acquire novel traits conferring a survival advantage. In the cariogenic pathogen Streptococcus mutans, DNA transformation is regulated by the competence stimulating peptide XIP (ComX-inducing peptide). The present study utilizes high-throughput RNA sequencing (RNAseq) to provide a greater understanding of how global gene expression patterns change in response to XIP. Overall, our work demonstrates that in S. mutans, XIP signaling induces a response that resembles the stringent response to amino acid starvation. We further identify a novel heat shock-responsive intergenic region with a potential role in competence shutoff. Together, our results provide further evidence that multiple stress response mechanisms are linked through the genetic competence signaling pathway in S. mutans. In the cariogenic Streptococcus mutans, competence development is regulated by the ComRS signaling system comprised of the ComR regulator and the ComS prepeptide to the competence signaling peptide XIP (ComX-inducing peptide). Aside from competence development, XIP signaling has been demonstrated to regulate cell lysis, and recently, the expression of bacteriocins, small antimicrobial peptides used by bacteria to inhibit closely related species. Our study further explores the effect of XIP signaling on the S. mutans transcriptome. RNA sequencing revealed that XIP induction resulted in a global change in gene expression that was consistent with a stress response. An increase in several membrane-bound regulators, including HdrRM and BrsRM, involved in bacteriocin production, and the VicRKX system, involved in acid tolerance and biofilm formation, was observed. Furthermore, global changes in gene expression corresponded to changes observed during the stringent response to amino acid starvation. Effects were also observed on genes involved in sugar transport and carbon catabolite repression and included the levQRST and levDEFG operons. Finally, our work identified a novel heat shock-responsive intergenic region, encoding a small RNA, with a potential role in competence shutoff. IMPORTANCE Genetic competence provides bacteria with an opportunity to increase genetic diversity or acquire novel traits conferring a survival advantage. In the cariogenic pathogen Streptococcus mutans, DNA transformation is regulated by the competence stimulating peptide XIP (ComX-inducing peptide). The present study utilizes high-throughput RNA sequencing (RNAseq) to provide a greater understanding of how global gene expression patterns change in response to XIP. Overall, our work demonstrates that in S. mutans, XIP signaling induces a response that resembles the stringent response to amino acid starvation. We further identify a novel heat shock-responsive intergenic region with a potential role in competence shutoff. Together, our results provide further evidence that multiple stress response mechanisms are linked through the genetic competence signaling pathway in S. mutans.