Concentrated collagen hydrogels as dermal substitutes
Concentrated collagen hydrogels as dermal substitutes
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DOI：
10.1016/j.biomaterials.2009.09.073
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发表时间：
2010-01-01
期刊：
影响因子：
14
通讯作者：
Giraud-Guille, Marie Madeleine
中科院分区：
文献类型：
--
作者：
Helary, Christophe;Bataille, Isabelle;Giraud-Guille, Marie Madeleine
胶原蛋白水凝胶最初似乎有希望用于皮肤修复。不幸的是，它们广泛的收缩和它们差的机械性能构成了它们作为永久移植物的主要缺点。目前的研究已经调查了一种方法来纠正这些缺点，增加胶原蛋白浓度在受控条件下。获得1.5、3和5 mg/ml的浓缩胶原水凝胶（CCH）。在培养物中评价升高的胶原蛋白浓度对收缩、细胞生长和重塑活性的影响21天。随后，评估CCH和正常胶原蛋白水凝胶（NCH）的体内整合。与NCH相比，CCH收缩延迟且较小。在第21天，3 mg/ml的CCH的表面积比NCH的表面积重要18倍。无论初始成纤维细胞密度如何，CCH有利于细胞生长，在第21天达到初始细胞数的约10倍; NCH中细胞增殖受到抑制。明胶酶A活性出现较低的CCH内NCH。大鼠体内研究显示，植入后15天NCH完全水解。相比之下，30天后仍存在3 mg/ml的CCH。此外，CCH显示细胞定植，新生血管形成，没有严重的炎症反应。我们的研究结果表明，浓缩的胶原蛋白水凝胶可以被认为是新的候选人的真皮替代品，因为它们是易于处理，不急剧收缩，有利于细胞生长，并可以快速集成在体内。(C)2009爱思唯尔有限公司保留所有权利。
Collagen hydrogels first appeared promising for skin repair. Unfortunately, their extensive contraction and their poor mechanical properties constituted major disadvantages toward their utilization as permanent graft. The present study has investigated a way to correct these drawbacks by increasing the collagen concentration in controlled conditions. Concentrated collagen hydrogels (CCH) at 1.5, 3 and 5 mg/ml were obtained. The effect of raised collagen concentration on contraction, cell growth and remodeling activities was evaluated for 21 days in culture. Subsequently, in vivo integration of CCH and normal collagen hydrogels (NCH) was assessed. Compared to NCH, CCH contraction was delayed and smaller. At day 21, surface area of CCH at 3 mg/ml was 18 times more important than that of NCH. Whatever the initial fibroblast density, CCH favored cell growth that reached about 10 times the initial cell number at day 21; cell proliferation was inhibited in NCH. Gelatinase A activities appeared lower in CCH than within NCH. In vivo studies in rats revealed a complete hydrolysis of NCH 15 days after implantation. In contrast, CCH at 3 mg/ml was still present after 30 days. Moreover, CCH showed cell colonization, neovascularization and no severe inflammatory response. Our results demonstrate that concentrated collagen hydrogels can be considered as new candidates for dermal substitution because they are is easy to handle, do not contract drastically, favor cell growth, and can be quickly integrated in vivo. (C) 2009 Elsevier Ltd. All rights reserved.