Physical map-based sizes of the centromeric regions of Arabidopsis thaliana chromosomes 1, 2, and 3
Physical map-based sizes of the centromeric regions of Arabidopsis thaliana chromosomes 1, 2, and 3
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DOI：
10.1093/dnares/9.4.117
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发表时间：
2002-08-31
期刊：
影响因子：
4.1
通讯作者：
Kotani, H
中科院分区：
文献类型：
--
作者：
Hosouchi, T;Kumekawa, N;Kotani, H
拟南芥染色体1、2、3的着丝粒区大小在限制性分析的基础上通过构建物理图谱确定。由于报道的着丝粒区由于高度重复的序列而在中间存在较大的间隙，因此从报道的侧翼区域序列和本工作新分离的BAC和YAC克隆序列中制备了合适的南方杂交探针，并使用低甲基化菌株（ddm1）的DNA进行了限制性内切分析。遗传定义的着丝粒区域的大小分别为9兆碱基（Mb）、4.2 Mb和4.1 Mb（染色体1，从标记T22C23-t7到T3P8-sp6；染色体2，从标记F5J15-sp6到T15D9；染色体3，从标记T9G9-sp6到T15M14； G. P. Copenhaver等）。科学，286,2468-2479,1999)。通过结合先前估计的4号和5号染色体的着丝粒区大小和报道的拟南芥基因组序列数据，估计拟南芥的总基因组大小约为146.0 Mb。
The sizes of the centromeric regions of Arabidopsis thaliana chromosomes 1, 2, and 3 were determined by construction of their physical maps on the basis of restriction analysis. As the reported centromeric regions contain large gaps in the middle due to highly repetitive sequences, appropriate probes for Southern hybridization were prepared from the sequences reported for the flanking regions and from the sequences of BAC and YAC clones newly isolated in this work, and restriction analysis was performed using DNA of a hypomethylated strain (ddm1). The sizes of the genetically defined centromeric regions were deduced to be 9 megabases (Mb), 4.2 Mb and 4.1 Mb, respectively (chromosome 1, from markers T22C23-t7 to T3P8-sp6; chromosome 2, from F5J15-sp6 to T15D9; chromosome 3, from T9G9-sp6 to T15M14; G. P. Copenhaver et al. Science, 286, 2468-2479, 1999). By combining the sizes of the centromeric regions previously estimated for chromosomes 4 and 5 and the sequence data reported for the A. thaliana genome, the total genome size of A. thaliana was estimated to be approximately 146.0 Mb.