Fluorescent immunohistochemistry and in situ hybridization analysis of pancreas.
Fluorescent immunohistochemistry and in situ hybridization analysis of pancreas.
复制标题
胰腺的荧光免疫组织化学和原位杂交分析。
DOI：
10.1007/978-1-59745-448-3_13
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Crooks,GayM
中科院分区：
文献类型：
--
作者：
Wang,Xiuli;Ge,Shundi;Crooks,GayM
To facilitate the immunological reaction of antibodies with antigens in fixed tissues, it is necessary to unmask or retrieve the antigens through pretreatment of the specimens. However, adjustment of heat-induced antigen retrieval is always required for different tissues and antigens. Using a low-power antigen retrieval technique, with appropriate dilution of antibodies, we successfully immunostained key antigens in the pancreas such as insulin, PDX-1, glucagon, cytokeratin, and CD31, which have presented a particular challenge for investigators in the past, because of the rapid autodigestion and high nonspecific antibody binding in the tissue. Satisfactory results were obtained when immunohistochemistry and fluorescence in situ hybridization analysis were combined in the same slides.
