Protective effects of desipramine on alveolar bone in experimental periodontitis.
Protective effects of desipramine on alveolar bone in experimental periodontitis.
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去丙胺对实验牙周炎中肺泡骨的保护作用。
DOI：
10.1002/jper.19-0569
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发表时间：
2020-12
影响因子：
4.3
通讯作者：
Rosalen, Pedro L.
中科院分区：
文献类型：
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作者：
Branco-de-Almeida, Luciana S.;Franco, Gilson C. N.;Castro, Myrella L.;Vieira, Mayana S.;Galvao-Moreira, Leonardo, V;Cortelli, Sheila C.;Anbinder, Ana L.;Kawai, Toshihisa;Rosalen, Pedro L.
地昔帕明是一种具有免疫调节活性的三环类抗抑郁药，其对结扎引起的牙周炎的影响尚待研究。因此，对其对牙槽骨吸收、牙龈胶原含量和关键炎症介质的作用进行了分析。总共60只雄性Wistar大鼠被随机分为三组：i)对照：用媒介物（盐水）处理的未结扎的大鼠； ii)结扎：患有结扎诱发的牙周炎的大鼠，用媒介物治疗； iii)结扎线+地昔帕明：用地昔帕明（媒介物中20mg/kg/天）治疗患有结扎诱发的牙周炎的大鼠。在插入结扎线（或对照组不插入结扎线）和治疗后 3 或 15 天收集下颌骨和牙龈组织。使用HE或天狼星红染色对牙槽骨吸收和牙龈胶原纤维进行组织学分析。通过逆转录聚合酶链反应获得牙龈白细胞介素（IL）-1β、诱导型一氧化氮合酶（iNOS）、环氧合酶（COX）-2、基质金属蛋白酶（MMP）-9和金属蛋白酶组织抑制剂（TIMP）-1的mRNA表达量。通过酶谱分析MMP-9活性。结扎+地昔帕明组牙槽骨丢失明显减少（P<0.05），而牙龈胶原蛋白降解与结扎组相似（P>0.05）。与单独使用载体相比，结扎组中地昔帕明给药下调了 IL-1β、iNOS、COX-2 和 TIMP-1 的 mRNA 表达（P<0.05）。结扎牙周炎大鼠MMP-9表达及MMP-9/TIMP-1比值相似（P>0.05）；然而，地昔帕明治疗组的 MMP-9 活性较低（P<0.05）。组织学观察到，地昔帕明给药减少了牙槽骨丢失，并调节了结扎引起的牙周炎大鼠的关键骨重塑和炎症介质。
Desipramine is a tricyclic antidepressant with immune-modulatory activity, whose effects on ligature-induced periodontitis are yet to be investigated. Hence, its actions on alveolar bone resorption, gingival collagen content and key inflammatory mediators were herewith analyzed. A total of 60 male Wistar rats were randomly assigned into three groups: i) control: rats without ligature treated with vehicle (saline); ii) ligature: rats with ligature-induced periodontitis treated with vehicle; iii) ligature + desipramine: rats with ligature-induced periodontitis treated with desipramine (20 mg/kg/day in vehicle). Mandibles and gingival tissues were collected 3 or 15 days after ligature insertion (or no ligature insertion for controls) and treatments. Alveolar bone resorption and gingival collagen fibers were histologically analyzed using either HE or picrosirius red staining. Gingival mRNA expressions of interleukin (IL)-1β, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction. MMP-9 activity was analyzed by zymography. Alveolar bone loss was significantly reduced in the ligature + desipramine group (P<0.05), whereas gingival collagen degradation was similar to the ligature group (P>0.05). Desipramine administration downregulated mRNA expressions of IL-1β, iNOS, COX-2 and TIMP-1 when compared to vehicle alone in the ligature group (P<0.05). MMP-9 expression and MMP-9/TIMP-1 ratio were similar among rats with ligature-induced periodontitis (P>0.05); however, MMP-9 activity was lower in the group treated with desipramine (P<0.05). Desipramine administration reduced alveolar bone loss as histologically observed, and modulated key bone remodeling and inflammatory mediators in rats with ligature-induced periodontitis.