Normal human primary fibroblasts undergo apoptosis in three-dimensional contractile collagen gels
Normal human primary fibroblasts undergo apoptosis in three-dimensional contractile collagen gels
复制标题
DOI：
10.1046/j.1523-1747.1998.00095.x
复制
发表时间：
1998-02-01
影响因子：
6.5
通讯作者：
Sollberg, S
中科院分区：
文献类型：
--
作者：
Fluck, J;Querfeld, C;Sollberg, S
在皮肤伤口愈合过程中，在体内观察到原代成纤维细胞的凋亡，预计也会发生在其他器官中;然而，诱导成纤维细胞凋亡的环境信号和细胞-基质相互作用对细胞凋亡调控的推定影响仍有待确定。在这里，我们提供的纤维胶原蛋白在这一过程中的作用的证据，并证明，正常的人原代成纤维细胞包埋在收缩性胶原蛋白凝胶进行细胞凋亡所示的细胞质组蛋白相关的DNA片段的外观开始在第1天的文化与高峰约2-4天。这种诱导原代成纤维细胞凋亡似乎是特异性的收缩性胶原凝胶，因为既没有观察到原代成纤维细胞的凋亡细胞培养皿上或塑料盘上涂有胶原，也没有观察到细胞接种在锚定的胶原凝胶或收缩性纤维蛋白凝胶。因此，我们得出结论，一个独特的环境，如收缩胶原基质决定了正常的原代成纤维细胞凋亡的易感性。
Apoptosis of primary fibroblasts was observed in vivo during wound healing in skin and is expected to occur in other organs as well; however, the environmental signal for induction of apoptosis in fibroblasts and the putative influence of cell-matrix interactions on the regulation of apoptosis remain to be identified. Here we provide evidence for the role of fibrillar collagen in this process, and demonstrate that normal human primary fibroblasts embedded in contractile collagen gels undergo apoptosis as shown by the appearance of cytoplasmatic histone-associated DNA fragments starting at day 1 of culture with a peak around days 2-4. This induction of apoptosis in primary fibroblasts seems to be specific for contractile collagen gels, because apoptosis of primary fibroblasts was neither observed in cells grown on culture dishes or on plastic dishes coated with collagen, nor observed in cells seeded in either anchored collagen gels or contractile fibrin gels. We therefore conclude that a distinct environment such as a contractile collagen matrix determines the susceptibility of normal primary fibroblasts to apoptosis.