GSTM1 modulation of IL-8 expression in human bronchial epithelial cells exposed to ozone.
GSTM1 modulation of IL-8 expression in human bronchial epithelial cells exposed to ozone.
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DOI：
10.1016/j.freeradbiomed.2011.05.006
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发表时间：
2011-07-15
影响因子：
7.4
通讯作者：
Peden, David B.
中科院分区：
文献类型：
--
作者：
Wu, Weidong;Doreswamy, Vinod;Diaz-Sanchez, David;Samet, James M.;Kesic, Matt;Dailey, Lisa;Zhang, Wenli;Jaspers, Ilona;Peden, David B.
Exposure to the major air pollutant ozone can aggravate asthma and other lung diseases. Our recent study in human volunteers has shown that the glutathione S-transferase mu 1 (GSTM1) null genotype is associated with increased airway neutrophilic inflammation induced by inhaled ozone. The aim of this study was to examine the effect of GSTM1 modulation on interleukin 8 (IL-8) production in ozone-exposed human bronchial epithelial cells (BEAS-2B) and the underlying mechanisms. Exposure of BEAS-2B cells to 0.4 ppm ozone for 4 h significantly increased IL-8 release with a modest reduction in intracellular reduced glutathione (GSH). Ozone exposure induced reactive oxygen species (ROS) production and NFκB activation. Pharmacological inhibition of NFκB activation or mutation of IL-8 promoter at κB-binding site significantly blocked ozone-induced IL-8 production or IL-8 transcriptional activity, respectively. Knockdown of GSTM1 in BEAS-2B cells enhanced ozone-induced NFκB activation and IL-8 production. Consistently, ozone-induced overt increase in IL-8 production was detected in GSTM1-null primary human bronchial epithelial cells. In addition, supplementation with reduced GSH inhibited ozone-induced ROS production, NFκB activation and IL-8 production. Taken together, GSTM1 deficiency enhances ozone-induced IL-8 production, which is mediated by generated ROS and subsequent NFκB activation in human bronchial epithelial cells.
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影响因子：
3.8
作者：
Chen, LC;Qu, QS
通讯作者：
Qu, QS
DOI：
10.1164/rccm.200305-684oc
发表时间：
2003-11-15
影响因子：
24.7
作者：
David, GL;Romieu, I;London, SJ
通讯作者：
London, SJ
影响因子：
14.2
作者：
Alexis, Neil E.;Zhou, Haibo;Peden, David B.
通讯作者：
Peden, David B.
影响因子：
6.1
作者：
Bosson, J;Stenfors, N;Blomberg, A
通讯作者：
Blomberg, A
DOI：
10.1164/ajrccm.162.4.9912041
发表时间：
2000-10-01
影响因子：
24.7
作者：
Avissar, NE;Reed, CK;Finkelstein, JN
通讯作者：
Finkelstein, JN