In vitro dissolution of melt-derived 45S5 and sol-gel derived 58S bioactive glasses
In vitro dissolution of melt-derived 45S5 and sol-gel derived 58S bioactive glasses
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DOI：
10.1002/jbm.10207
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发表时间：
2002-08-01
期刊：
影响因子：
--
通讯作者：
Hench, LL
中科院分区：
文献类型：
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作者：
Sepulveda, P;Jones, JR;Hench, LL
使用熔融衍生的45 S5和溶胶-凝胶衍生的58 S生物活性玻璃粉末，在体外研究了粉末类型、粒度（5-20 μ m; 90-300 μ m; 90-710 μ m）和溶解介质类型对生物活性玻璃溶解行为的影响。在37 ℃、动态条件（1 Hz）下，在模拟体液和基于α-MEM的细胞培养基中进行溶出度研究，持续时间为30 min、1、2、4、8、17和22 h。使用电感耦合等离子体分析来评估从玻璃中溶解的元素的浓度。使用傅里叶变换红外光谱法分析反应的粉末的生物活性，以观察在表面上磷酸钙层的形成。熔融衍生的45 S5玻璃粉末的无孔表面表现出比58 S凝胶玻璃粉末更低的溶解速率和表面层形成速率。与模拟体液相比，两种类型的粉末在培养基中的溶出速率均较低，并且随着粒度的减小而增加。因此，粒度范围、玻璃类型和粉末体积分数可用作控制活性离子释放速率的手段，所述活性离子刺激基因表达和细胞反应以用于组织增殖和修复。(C)2002 Wiley Periodicals，Inc.
Effects of powder type, particle size (5-20 mum; 90-300 mum; 90-710 mum), and type of dissolution medium on the dissolution behavior of bioactive glasses were investigated in vitro using melt-derived 45S5 and sol-gel derived 58S bioactive glass powders. Dissolution studies were performed in simulated body fluid and in alpha-MEM based cell culture medium at 37degreesC under dynamic conditions (1 Hz) for periods of 30 min, 1, 2, 4, 8, 17, and 22 h. The concentrations of elements dissolved from the glasses were evaluated using inductively coupled plasma analysis. The reacted powders were analyzed for bioactivity using Fourier transform infrared spectrometry to observe the formation of a calcium phosphate layer on the surface. The non-porous surfaces of melt-derived 45S5 glass powders exhibited lower dissolution rates and rate of surface layer formation than 58S gel-glass powders. The rates of dissolution for both types of powders were lower in culture medium, compared to simulated body fluid, and increased as the particle size decreased. Thus, particle size range, glass type, and powder volume fraction can be used as a means to control the release rate of active ions that stimulate the gene expression and cellular response for tissue proliferation and repair. (C) 2002 Wiley Periodicals, Inc.