Androgen-independent cancer progression and bone metastasis in the LNCaP model of human prostate cancer.
Androgen-independent cancer progression and bone metastasis in the LNCaP model of human prostate cancer.
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发表时间：
1994-05
期刊：
影响因子：
11.2
通讯作者：
G. Thalmann;P. Anezinis;Shih-Ming Chang;H. Zhau;E. Kim;V. Hopwood;S. Pathak;A. C. Eschenbach;L. Chung
中科院分区：
文献类型：
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作者：
G. Thalmann;P. Anezinis;Shih-Ming Chang;H. Zhau;E. Kim;V. Hopwood;S. Pathak;A. C. Eschenbach;L. Chung
我们的实验室以前曾报道过从去势和完整的无胸腺雄性小鼠的LNCaP肿瘤中获得LNCaP细胞亚系。这些LNCaP亚系在致瘤性和雄激素依赖性方面不同于亲本系。本文证明，这些亚系之一获得转移潜力。当接种时，或原位转移，C4-2亚系转移至淋巴结和骨，发生率为11- 50%。有趣的是，骨转移的发生率在去势的男性宿主中高于完整的男性宿主。我们评估了来自转移至淋巴结和骨的C4-2肿瘤的LNCaP亚系的染色体、免疫组织化学和生化特征。细胞遗传学分析表明，所有亚系都是人类的，与亲本LNCaP细胞共享共同的标记染色体。这种实验性的人类前列腺癌模型可能首次允许研究人类前列腺癌转移的分子机制。
Our laboratory has previously reported on the derivation of LNCaP cell sublines from LNCaP tumors maintained in castrated and intact athymic male mice. These LNCaP sublines differ from the parental line in tumorigenicity and androgen dependence. This paper demonstrates that one of these sublines acquired metastatic potential. When inoculated either s.c. or orthotopically, the C4-2 subline metastasized to the lymph node and bone with an incidence of 11-50%. Interestingly, the incidence of osseous metastasis was higher in castrated than in intact male hosts. We evaluated the chromosomal, immunohistochemical, and biochemical characteristics of the LNCaP sublines derived from C4-2 tumors that metastasized to the lymph node and bone. Cytogenetic analysis showed that all sublines were human and shared common marker chromosomes with the parental LNCaP cells. This experimental human prostate cancer model may permit, for the first time, the study of the molecular mechanisms underlying human prostate cancer metastasis.