N-glycomic changes in hepatocellular carcinoma patients with liver cirrhosis induced by hepatitis B virus
N-glycomic changes in hepatocellular carcinoma patients with liver cirrhosis induced by hepatitis B virus
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DOI：
10.1002/hep.21855
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发表时间：
2007-11-01
期刊：
影响因子：
13.5
通讯作者：
Chen, Cuiying
中科院分区：
文献类型：
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作者：
Liu, Xue-En;Desmyter, Liesbeth;Chen, Cuiying
我们评价了血清N-葡聚糖指纹图谱作为诊断乙肝病毒(乙肝病毒)所致肝硬变患者肝细胞癌的一种工具。对450例合并或不合并肝细胞癌的肝纤维化或肝硬变患者进行了研究。通过甲胎蛋白(AFP)分析、超声和/或计算机断层扫描诊断为肝细胞癌，并进行组织学研究。用基于DNA测序仪的碳水化合物分析图谱技术测定血清蛋白的N-糖链图谱。在这项研究中，我们发现，在肝细胞癌患者中，分支α(1，3)岩藻糖基化的三天线多糖比在肝硬变患者、纤维化患者和健康献血者中更丰富，而在肝硬变患者中，对分的核心α(1，6)岩藻糖基化的二天线多糖升高。这两种多糖的浓度和9号峰与7号峰的对数比(更名为GlycoHCCTest)与肿瘤分期有关。此外，对于从肝硬变患者中筛选肝细胞癌患者，GlycoHCCTest的总体敏感性和特异性与AFP非常相似。结论：本研究表明分支α(1，3)岩藻糖化的糖链与肝细胞癌的发生有关。血清N-葡聚糖谱是一种有前途的无创检测肝硬变患者肝细胞癌的方法，在诊断乙肝病毒感染的肝硬变患者肝细胞癌方面可作为甲胎蛋白的有价值的补充。它在肝硬变和肝细胞癌患者的筛查、随访和治疗中的应用应该得到进一步的评价。
We evaluated the use of blood serum N-glycan fingerprinting as a tool for the diagnosis of hepatocellular carcinoma (HCC) in patients with cirrhosis induced by hepatitis B virus (HBV). A group of 450 HBV-infected patients with liver fibrosis or cirrhosis with or without HCC were studied. HCC was diagnosed by a-fetoprotein (AFP) analysis, ultrasonography, and/or computed tomography and was studied histologically. N-glycan profiles of serum proteins were determined with DNA sequencer-based carbohydrate analytical profiling technology. In this study, we found that a branch alpha(1,3)fucosylated triantennary glycan was more abundant in patients with HCC than in patients with cirrhosis, patients with fibrosis, and healthy blood donors, whereas a bisecting core alpha(1,6)-fucosylated biantennary glycan was elevated in patients with cirrhosis. The concentration of these 2 glycans and the log ratio of peak 9 to peak 7 (renamed the GlycoHCCTest) were associated with the tumor stage. Moreover, for screening patients with HCC from patients with cirrhosis, the overall sensitivity and specificity of the GlycoHCCTest were very similar to those of AFP. Conclusion: This study indicates that a branch alpha(1,3)-fucosylated glycan is associated with the development of HCC. The serum N-glycan profile is a promising noninvasive method for detecting HCC in patients with cirrhosis and could be a valuable supplement to AFP in the diagnosis of HCC in HBV-infected patients with liver cirrhosis. Its use for the screening, follow-up, and management of patients with cirrhosis and HCC should be evaluated further.