ON EXISTENCE OF SPECTRALLY DISTINCT CLASSES OF FLAVOPROTEIN SEMIQUINONES . A NEW METHOD FOR QUANTITATIVE PRODUCTION OF FLAVOPROTEIN SEMIQUINONES
ON EXISTENCE OF SPECTRALLY DISTINCT CLASSES OF FLAVOPROTEIN SEMIQUINONES . A NEW METHOD FOR QUANTITATIVE PRODUCTION OF FLAVOPROTEIN SEMIQUINONES
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DOI：
10.1021/bi00874a016
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发表时间：
1966-01-01
期刊：
影响因子：
2.9
通讯作者：
PALMER, G
中科院分区：
文献类型：
--
作者：
MASSEY, V;PALMER, G
介绍了一种将黄酮类蛋白转化为半醌类化合物的新方法。这包括在EDTA[四乙酸乙二胺]存在下的厌氧光照射。用这种技术对一些简单的黄酮类蛋白进行了调查，得出结论，黄酮类蛋白自由基存在两种不同的类别，根据它们的光学吸收特性很容易区分。对于葡萄糖氧化酶，两种类型的自由基都存在，其浓度取决于ph值。这些结果表明，两种不同的光谱物种可能是由于中性的？黄素辅酶辅基的阴离子自由基形式。
A new method for the conversion of flavoproteins to the semiquinoid form is described. This consists of anaerobic photoirradiation in the presence of EDTA [ethylenediamine tetraacetate]. A survey of a number of simple flavoproteins with this technique leads to the conclusion that there exist 2 different classes of flavoprotein free radicals, which are very readily distinguished on the basis of their optical absorption properties. With one enzyme, glucose oxidase, both types of radical are found, the concentrations depending on the pH. These results suggest that the 2 different spectral species may be due to the neutral ?nd anionic radical forms of the flavin coenzyme prosthetic groups.