Membrane oestrogen receptors on rat pituitary tumour cells: immuno-identification and responses to oestradiol and xenoestrogens.
Membrane oestrogen receptors on rat pituitary tumour cells: immuno-identification and responses to oestradiol and xenoestrogens.
复制标题
大鼠垂体肿瘤细胞上的膜雌激素受体：免疫鉴定以及对雌二醇和异雌激素的反应。
DOI：
10.1111/j.1469-445x.1999.01903.x
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发表时间：
1999
影响因子：
2.7
通讯作者：
Gametchu,B
中科院分区：
文献类型：
--
作者：
Watson,CS;Campbell,CH;Gametchu,B
我们的实验室已经在 GH3/B6 大鼠垂体肿瘤细胞系和几个亚系上鉴定出质膜雌激素受体，这些受体对雌激素产生快速（几分钟内）、非基因组反应。雌激素受体通过与九种不同抗体 (Ab) 的结合而被识别，这些抗体一起识别雌激素受体-α 上的至少七个表位。 GH3/B6/F10 细胞是一种富含雌激素受体的膜亚系，响应 10 nM 雌二醇而升高细胞内钙水平。这些细胞中催乳素的释放由 1 pM 和 1 nM 雌二醇和己烯雌酚 (DES) 触发。当向细胞施加 1 nM 雌二醇、10 nM 己烯雌酚或 10 nM 壬基酚时，膜雌激素受体-α 免疫细胞化学信号迅速消失（3 分钟）并重新出现（12-15 分钟）。这表明雌激素和异种雌激素都可以利用这种替代途径发挥雌激素作用。迄今为止，异种雌激素在基因组测定系统中显示出较弱的作用，现在应该重新测试其在引发膜引发的雌激素反应方面的活性。
Our laboratory has identified plasma membrane oestrogen receptors on a GH3/B6 rat pituitary tumour cell line and several sublines which produce rapid (within minutes), non-genomic responses to oestrogens. Oestrogen receptors have been identified by their binding to nine different antibodies (Abs) which together recognize at least seven epitopes on the oestrogen receptor-α. GH3/B6/F10 cells, a membrane oestrogen receptor-enriched subline, elevate intracellular calcium levels in response to 10 nM oestradiol. Prolactin release in these cells is triggered by both 1 pM and 1 nM oestradiol and diethylstilbestrol (DES). A membrane oestrogen receptor-α immunocyto-chemical signal rapidly disappears (at 3 min) and reappears (at 12-15 min) when 1 nM oestradiol, 10 nM diethylstilbestrol, or 10 nM nonylphenol is applied to the cells. This suggests that both oestrogens and xenoestrogens can utilize this alternative pathway for oestrogenic action. Xenoestrogens, which have so far shown weak effects in genomic assay systems, should now be retested for activity in eliciting membrane-initiated oestrogenic responses.