Biocompatible Composite Microspheres of Chitin/Ordered Mesoporous Carbon CMK3 for Bilirubin Adsorption and Cell Microcarrier Culture
Biocompatible Composite Microspheres of Chitin/Ordered Mesoporous Carbon CMK3 for Bilirubin Adsorption and Cell Microcarrier Culture
复制标题
用于胆红素吸附和细胞微载体培养的生物相容性甲壳素/有序介孔碳CMK3复合微球
DOI：
10.1002/mabi.202100412
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发表时间：
2022
影响因子：
4.6
通讯作者：
Qifa Ye
中科院分区：
文献类型：
--
作者：
Pengpeng Yue;Biao Chen;Xiaoyan Lv;Yongkang Zou;Hankun Cao;Yongsheng Ma;Lizhe Wang;Zhongzhong Liu;Yiran Zheng;Bo Duan;Shuangquan Wu;Qifa Ye
血液中多余的胆红素会引起严重肝病患者的严重疾病。血液灌流是去除多余胆红素的有效方法，但现有吸附材料的吸附效率低、生物相容性差，限制了其应用。本研究成功制备了甲壳素/有序介孔碳CMK3 （Ch/CMK3）微球。表征实验结果表明，这些复合微球具有多层多孔纳米纤维结构，具有极大的比表面积（300.19 m2 - 1）和大孔径。值得注意的是，Ch/CMK3微球在磷酸盐缓冲溶液（PBS）中具有较高的胆红素吸附能力（228.19 mg g−1），在高胆红素血症家兔血浆中具有出色的胆红素去除率（76.78%±4.40%），且不影响蛋白质成分。更重要的是，Ch/CMK3微球对其他血液成分没有影响，对小鼠没有细胞毒性，也没有全身毒性。细胞共培养实验表明，微球可以提供一个三维空间来促进细胞的粘附、增殖和营养交换。这些Ch/CMK3微球具有较强的胆红素吸附能力和良好的生物相容性，在血液灌流、细胞微载体和3D组织工程等生物医学应用中具有广阔的应用前景。
Extra bilirubin in the blood can provoke serious illness in patients with severe liver disease. Hemoperfusion is an effective method to remove the extra bilirubin, but its application is limited by the low adsorption efficiency and poor biocompatibility of available adsorbent materials. In this study, chitin/ordered mesoporous carbon CMK3 (Ch/CMK3) microspheres are successfully prepared. Results of characterization experiments indicated that these composite microspheres possess a multilayered porous nanofibrous structure with an extremely large specific surface area (300.19 m2g−1) and large pore size. Notably, the Ch/CMK3 microspheres demonstrated a high bilirubin adsorption capacity (228.19 mg g−1) in phosphate buffer solution (PBS), and an outstanding bilirubin removal ratio (76.78% ± 4.40%) in the plasma of rabbits with hyperbilirubinemia without affecting the protein components. More importantly, the Ch/CMK3 microspheres showed no effect on other blood components, no cytotoxicity, and no systemic toxicity to mice. Cell co‐culture experiments revealed that the microspheres can provide a 3‐dimensional (3D) space to promote cell adhesion, proliferation, and nutrient exchange. These Ch/CMK3 microspheres featuring a strong ability for bilirubin adsorption and good biocompatibility can be a promising candidate in biomedical applications such as hemoperfusion, cell microcarrier, and 3D tissue engineering.