A new amperometric enzyme electrode for galactose determination
A new amperometric enzyme electrode for galactose determination
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DOI：
10.1016/s0141-0229(01)00452-5
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发表时间：
2002-01-08
影响因子：
3.4
通讯作者：
Yildiz, A
中科院分区：
文献类型：
--
作者：
Gülce, H;Ataman, I;Yildiz, A
将半乳糖氧化酶固定于包覆在Pt电极表面的聚乙烯二茂铁基质中，制备了一种新的测定半乳糖的酶电极。酶电极在+0.70V vs SCE恒定电位下的电流响应测量。考察了半乳糖浓度和温度对酶电极反应的影响。响应时间为30 ~ 40 s，线性工作部分的上限为半乳糖浓度40.0 mM。(C) 2002爱思唯尔科学有限公司版权所有。
A new enzyme electrode for the determination of galactose was developed by immobilizing galactose oxidase in polyvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response of the enzyme electrode was measured at a constant potential of +0.70V vs SCE. The effects of galactose concentration and temperature on the response of the enzyme electrode were investigated. The response time was found to be 30-40 s and the upper limit of the linear working portion was found to be 40.0 mM galactose concentration. (C) 2002 Elsevier Science Inc. All rights reserved.