Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice
Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice
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DOI：
10.1155/2011/532180
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发表时间：
2011-01-01
影响因子：
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通讯作者：
Mizuno, Masashi
中科院分区：
文献类型：
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作者：
Bouike, Go;Nishitani, Yosuke;Mizuno, Masashi
为阐明巴西蘑菇提取物（ABME）抗过敏作用的机制，采用体内过敏模型和体外肠道模型。在致敏过程中，ABME组血清IgE水平显著降低。在ABME组中，由OVA再刺激的脾细胞产生的白细胞介素（IL）-4和-5显著降低，并且抗CD 3 β/CD 28抗体处理也降低了IL-10、-4和-5的产生，并且增加了IFN-γ的产生。这些结果表明，口服ABME可改善Th 1/Th 2平衡。此外，由Caco-2细胞和来自OT-II小鼠的脾细胞或RAW 264.7细胞构建的共培养系统表明，ABME处理显著增加IFN-γ的产生。因此，可以得出结论，ABME的抗过敏活性是由于上皮细胞激活巨噬细胞和促进幼稚T细胞分化为免疫中的Th 1细胞。
To clarify the mechanism of the antiallergic activity of Agaricus blazei Murill extract (ABME), the present paper used an in vivo allergy model and an in vitro intestinal gut model. During OVA sensitization, the serum IgE levels decreased significantly in ABME group. Interleukin (IL)-4 and -5 produced from OVA-restimulated splenocytes was significantly decreased, and anti-CD3 epsilon/CD28 antibody treatment also reduced IL-10, -4, and -5 production and increased IFN-gamma production in ABME group. These results suggest that oral administration of ABME improves Th1/Th2 balance. Moreover, a coculture system constructed of Caco-2 cells and splenocytes from OT-II mice or RAW 264.7 cells indicated that the significant increases in IFN-gamma production by ABME treatment. Therefore, it was concluded that the antiallergic activity of ABME was due to the activation of macrophages by epithelial cells and the promotion of the differentiation of naive T cells into Th1 cells in the immune.