Long noncoding RNA PANDAR inhibits the development of lung cancer by regulating autophagy and apoptosis pathways
Long noncoding RNA PANDAR inhibits the development of lung cancer by regulating autophagy and apoptosis pathways
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长链非编码RNA PANDAR通过调节自噬和凋亡途径抑制肺癌的发生
DOI：
10.7150/jca.45291
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发表时间：
2020
影响因子：
3.9
通讯作者：
Lu Jiachun
中科院分区：
文献类型：
--
作者：
Zhang Lan;Wang Yuanhang;Xia Shengyuan;Yang Lei;Wu Di;Zhou Yifeng;Lu Jiachun
工作背景：lncRNA在肺癌的发生发展中起重要作用，但lncRNA是否通过调节自噬和凋亡水平影响肺癌的发生发展，目前尚不清楚。在这里，我们研究了PANDAR在NSCLC中的作用。材料与方法：在276例肺癌组织中检测PANDAR及其可能的靶基因BECN 1的表达谱和临床应用。通过实际实验，探讨PANDAR在体外对非小细胞肺癌细胞增殖、凋亡和自噬的作用。结果如下：PANDAR在肺癌组织和肺癌细胞系中表达均较对照组下调（P均< 0.05），且与肿瘤分期有关（P < 0.05）。自噬相关基因BECN 1在肺癌组织中表达较正常组织明显下调（P < 0.01），PANDAR与BECN 1表达水平呈显著正相关（r = 0.789，P < 0.001）。因此，PANDAR的高表达增加了BECN 1的表达水平，并在体外损害了NSCLC细胞系的增殖。进一步研究表明PANDAR可以调节细胞自噬和凋亡水平。结论：这些结果表明lncRNA PANDAR是一种肿瘤抑制因子，可以通过上调BECN 1表达激活自噬和凋亡途径来抑制NSCLC细胞增殖。
Background: LncRNAs has been shown to play important roles in the progression of lung cancer, but it remains poorly understood whether lncRNAs affect the occurrence and development of lung cancer by regulating autophagy and apoptosis levels. Here, we investigated the roles of PANDAR in NSCLC. Materials and Methods: The expression profile and clinical application of PANDAR and its possible target gene BECN1 were tested in 276 cases of lung cancer tissues. Through some actual experiments, we explored functions of PANDAR about proliferation, apoptosis and autophagy of NSCLC cells in vitro. Results: PANDAR was found to downregulate both in lung cancer tissues and cell lines compared with corresponding controls (P < 0.05 for all), which was related to tumor stage (P < 0.05). Moreover, autophagy related gene BECN1 was also downregulated in lung cancer tissues comparison with normal tissues (P < 0.01), and there was a significant positive correlation between PANDAR and BECN1 levels (r = 0.789, P < 0.001). So, the high expression of PANDAR increased BECN1 expression levels and impaired the proliferation of NSCLC cell lines in vitro. Furthermore study showed PANDAR could regulate cell autophagy and apoptosis levels. Conclusion: These results indicated lncRNA PANDAR was a tumor suppressor and can inhibit NSCLC cell proliferation by activating autophagy and apoptosis pathways via upregulation of BECN1 expression.