Mechanism of transcriptional repression of E2F by the retinoblastoma tumor suppressor protein.
Mechanism of transcriptional repression of E2F by the retinoblastoma tumor suppressor protein.
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DOI：
10.1016/s1097-2765(00)80310-x
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发表时间：
1999-02
期刊：
影响因子：
16
通讯作者：
John F. Ross;Xuan Liu;B. Dynlacht
中科院分区：
文献类型：
--
作者：
John F. Ross;Xuan Liu;B. Dynlacht
The retinoblastoma tumor suppressor protein (pRB) is a transcriptional repressor, critical for normal cell cycle progression. We have undertaken studies using a highly purified reconstituted in vitro transcription system to demonstrate how pRB can repress transcriptional activation mediated by the E2F transcription factor. Remarkably, E2F activation became resistant to pRB-mediated repression after the establishment of a partial (TFIIA/TFIID) preinitiation complex (PIC). DNase I footprinting studies suggest that E2F recruits TFIID to the promoter in a step that also requires TFIIA and confirm that recruitment of the PIC by E2F is blocked by pRB. These studies suggest a detailed mechanism by which E2F activates and pRB represses transcription without the requirement of histone-modifying enzymes.
