TRANSCRIPTS FROM THE CELLULAR HOMOLOGS OF RETROVIRAL ONCOGENES - DISTRIBUTION AMONG CHICKEN TISSUES
TRANSCRIPTS FROM THE CELLULAR HOMOLOGS OF RETROVIRAL ONCOGENES - DISTRIBUTION AMONG CHICKEN TISSUES
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DOI：
10.1128/mcb.2.6.617
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发表时间：
1982-01-01
影响因子：
5.3
通讯作者：
BISHOP, JM
中科院分区：
文献类型：
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作者：
GONDA, TJ;SHEINESS, DK;BISHOP, JM
快速转化逆转录病毒的癌基因（v-onc基因）在正常细胞的基因组中具有同源基因（c-onc基因）。对c-myb、c-myc、c-erb和c-src 4个c-onc基因在多种鸡细胞和组织中的转录进行了表征和定量。电泳分析的聚腺苷酸化的RNA，然后转移到硝酸纤维素和杂交克隆的onc探针表明，c-myb，c-myc和c-src每个产生一个单一的成熟的转录本; c-erb产生多个转录本之间的丰度不同的细胞和组织。通过斑点杂交测定定量c-myb、c-myc、c-erb和c-src的转录。C-myc，c-erb和c-src的转录几乎可以检测到所有的细胞和组织检查; c-myb的转录只检测到一些造血细胞，但是，这些细胞属于几个不同的谱系。因此，在任何情况下，c-onc基因的表达都不限于单细胞谱系。c-myb的表达水平与所检测的组织和细胞的造血活性之间似乎存在相关性，这表明c-myb可能主要在未成熟的造血细胞中表达。对携带相应v-onc基因的病毒的靶细胞和组织中c-onc RNA水平的检查显示，对给定v-onc基因转化的易感性与同源c-onc基因的表达之间没有明显的直接或反向相关性。
The oncogenes (v-onc genes) of rapidly transforming retroviruses have homologs (c-onc genes) in the genomes of normal cells. Transcription from 4 c-onc genes, c-myb, c-myc, c-erb and c-src, was characterized and quantitated in a variety of chicken cells and tissues. Electrophoretic analysis of polyadenylated RNA, followed by transfer to nitrocellulose and hybridization to cloned onc probes showed that c-myb, c-myc and c-src each give rise to a single mature transcript; c-erb gives rise to multiple transcripts which vary in abundance among different cells and tissues. Transcription from c-myb, c-myc, c-erb and c-src was quantitated by a dot-blot hybridization assay. C-myc, c-erb and c-src transcription could be detected in nearly all cells and tissues examined; c-myb transcription was detected only in some hemopoietic cells; these cells, however, belong to several different lineages. Thus, in no case was expression of a c-onc gene restricted to a single cell lineage. There appeared to be a correlation between levels of c-myb expression and hemopoietic activity of the tissues and cells examined, which suggests that c-myb may be expressed primarily in immature hemopoietic cells. An examination of c-onc RNA levels in target cells and tissues for viruses carrying the corresponding v-onc genes revealed no obvious correlation, direct or inverse, between susceptibility to transformation by a given v-onc gene and expression of the homologous c-onc gene.