Mechanisms and biomedical implications of-1 programmed ribosome frameshifting on viral and bacterial mRNAs
Mechanisms and biomedical implications of-1 programmed ribosome frameshifting on viral and bacterial mRNAs
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DOI：
10.1002/1873-3468.13478
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发表时间：
2019-07-01
期刊：
影响因子：
3.5
通讯作者：
Rodnina, Marina V.
中科院分区：
文献类型：
--
作者：
Korniy, Natalia;Samatova, Ekaterina;Rodnina, Marina V.
一些蛋白质的表达是由于核糖体移框事件的结果，该事件由mRNA中的一个滑点和下游的二级结构元件促进。本文综述了近年来在IBV1a/1b、HIV-1Gag-Poll、SFV6K等几种病毒基因以及在大肠杆菌dna X中-1移码机制的研究进展。确切的移码途径取决于氨基酰-tRNAs的可用性：核糖体通常在tRNA移位过程中滑入-1-框架，但在氨酰-tRNA供应有限的情况下，也可以在解码过程中移码。不同的移码路线和更多的滑点使病毒能够保持其关键蛋白质的持续生产。TRNA池对移码很重要的这一新兴想法为开发抗病毒疗法提供了新的方向。
Some proteins are expressed as a result of a ribosome frameshifting event that is facilitated by a slippery site and downstream secondary structure elements in the mRNA. This review summarizes recent progress in understanding mechanisms of -1 frameshifting in several viral genes, including IBV 1a/1b, HIV-1 gag-pol, and SFV 6K, and in Escherichia coli dnaX. The exact frameshifting route depends on the availability of aminoacyl-tRNAs: the ribosome normally slips into the -1-frame during tRNA translocation, but can also frameshift during decoding at condition when aminoacyl-tRNA is in limited supply. Different frameshifting routes and additional slippery sites allow viruses to maintain a constant production of their key proteins. The emerging idea that tRNA pools are important for frameshifting provides new direction for developing antiviral therapies.