Analytical standards production for the analysis of pomegranate anthocyanins by HPLC
Analytical standards production for the analysis of pomegranate anthocyanins by HPLC
复制标题
高效液相色谱法分析石榴花青素的分析标准品的制备
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
S. Freitas
中科院分区：
文献类型：
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作者：
M. Santiago;A. Gouvêa;R. Godoy;R. Borguini;S. Pacheco;R. Nogueira;L. Nascimento;S. Freitas
Pomegranate (Punica granatum L.) is a fruit with a long medicinal history, especially due to its phenolic compounds content, such as the anthocyanins, which are reported as one of the most important natural antioxidants. The analysis of the anthocyanins by high performance liquid chromatography (HPLC) can be considered as an important tool to evaluate the quality of pomegranate juice. For research laboratories the major challenge in using HPLC for quantitative analyses is the acquisition of high purity analytical standards, since these are expensive and in some cases not even commercially available. The aim of this study was to obtain analytical standards for the qualitative and quantitative analysis of the anthocyanins from pomegranate. Five vegetable matrices (pomegranate flower, jambolan, jabuticaba, blackberry and strawberry fruits) were used to isolate each of the six anthocyanins present in pomegranate fruit, using an analytical HPLC scale with non-destructive detection, it being possible to subsequently use them as analytical standards. Furthermore, their identities were confirmed by high resolution mass spectrometry. The proposed procedure showed that it is possible to obtain analytical standards of anthocyanins with a high purity grade (98.0 to 99.9%) from natural sources, which was proved to be an economic strategy for the production of standards by laboratories according to their research requirements.
影响因子：
6.1
作者：
Lu QY;Zhang Y;Wang Y;Wang D;Lee RP;Gao K;Byrns R;Heber D
通讯作者：
Heber D