TRANSFORMED MAMMALIAN-CELLS SECRETE SPECIFIC PROTEINS AND PHOSPHOPROTEINS
TRANSFORMED MAMMALIAN-CELLS SECRETE SPECIFIC PROTEINS AND PHOSPHOPROTEINS
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DOI：
10.1016/0092-8674(79)90103-x
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
HYNES, RO
中科院分区：
文献类型：
--
作者：
SENGER, DR;WIRTH, DF;HYNES, RO
检测正常细胞和转化细胞分泌到培养液中的蛋白质。几种哺乳动物sp.转化的细胞系。[仓鼠、大鼠和小鼠]分泌的蛋白质在58,000道尔顿兆瓦范围内。这些蛋白质是免疫相关的，由亲代正常细胞系低水平分泌或根本不分泌。58K蛋白的分泌发生在DNA或RNA病毒转化和自发转化过程中。转化的细胞分泌相同大小范围的磷酸蛋白，但这些蛋白在免疫学上与上述58K蛋白不同。磷蛋白的大小因物种而异，与转化病毒无关。转化细胞培养上清液与γ-~(32)P-ATP标记大小相同的磷蛋白，表明在蛋白激酶和底物的培养液中存在。所有这三个特性(58K蛋白、磷酸蛋白、体外磷酸化)都与温度敏感病毒转化细胞的转化密切相关。这些结果的生物学意义尚不清楚，但这些结果可能与最近关于RNA肿瘤病毒编码的(磷酸)蛋白和蛋白激酶的数据有关，以及转化表型的分子基础。
The proteins secreted into the growth medium by normal and transformed cells were examined. Transformed cell lines from several mammalian sp. [hamster, rat and mouse] secrete proteins in the 58,000 dalton MW range. These proteins are immunologically related and are secreted at low levels or not at all by the parental normal cell lines. Secretion of the 58K proteins occurs with DNA or RNA virus transformation and with spontaneous transformation. The transformed cells secrete phosphoproteins in the same size range, but these are immunologically distinct from the 58K proteins mentioned above. The sizes of the phosphoproteins are species-specific and unrelated to the transforming virus. Incubation of conditioned media from transformed cell cultures with .gamma.-32P-ATP labels phosphoproteins of the same sizes, indicating the presence in the media of protein kinase and substrate. All 3 properties (58K protein, phosphoprotein, in vitro phosphorylation) are closely correlated with transformation in cells transformed by temperature-sensitive viruses. The biological implications of these results remain unknown, but the results may be relevant to recent data on the (phospho)proteins and protein kinase encoded by RNA tumor viruses and the molecular basis of the transformed phenotype.