Development of a Two-Step Multiplex PCR Assay for Typing of Capsular Polysaccharide Synthesis Gene Clusters of Streptococcus suis
Development of a Two-Step Multiplex PCR Assay for Typing of Capsular Polysaccharide Synthesis Gene Clusters of Streptococcus suis
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DOI：
10.1128/jcm.03411-13
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发表时间：
2014-05-01
影响因子：
9.4
通讯作者：
Takamatsu, Daisuke
中科院分区：
文献类型：
--
作者：
Okura, Masatoshi;lachance, ClauDe;Takamatsu, Daisuke
我们建立了一种实用、简便的两步多重聚合酶链式反应方法来辅助猪链球菌的血清学分型。该方法对几乎所有的血清型参考菌株和不同血清型的野毒株进行了准确的分型，还鉴定了一些血清学上不能分型的菌株的衣壳多糖合成基因簇的基因类型。
We developed a practical and easy two-step multiplex PCR assay to aid in serotyping of Streptococcus suis. The assay accurately typed almost all of the serotype reference strains and field isolates of various serotypes and also identified the genotypes of capsular polysaccharide synthesis gene clusters of some serologically nontypeable strains.