Fibronectin in basal cell epithelioma: sources and significance.
Fibronectin in basal cell epithelioma: sources and significance.
复制标题
基底细胞上皮瘤中的纤连蛋白：来源和意义。
DOI：
10.1111/1523-1747.ep12259701
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Clark,RA
中科院分区：
文献类型：
--
作者：
Grimwood,RE;Huff,JC;Harbell,JW;Clark,RA
结节性基底细胞上皮瘤 (BCE) 在肿瘤结节内和结节间质界面（基底膜区）均含有纤连蛋白。肿瘤结节内或周围的纤连蛋白可能源自肿瘤细胞、截留的基质或血浆。本研究旨在阐明 BCE 结节内纤连蛋白的来源。如果间质卡压发生到任何程度，肿瘤结节内的血友病因子 VIII:Ag 染色的血管应通过免疫荧光技术显而易见。同样，如果血浆蛋白沉积在 BCE 中，肿瘤结节应该用荧光素偶联的抗纤维蛋白原抗体染色。因此，用罗丹明缀合的抗人纤连蛋白和荧光素缀合的抗人因子VIII:Ag或荧光素标记的抗人纤维蛋白原对6个基底细胞上皮瘤进行双重标记。纤连蛋白沿肿瘤小叶边缘呈线性排列，并在肿瘤组织的中心部分呈细丝状沉积物。在任何肿瘤小叶中均未发现纤维蛋白原或因子 VIII:Ag 的证据。因子 VIII:Ag 以颗粒形式存在于肿瘤巢之间的血管壁内。使用兔抗人纤连蛋白和过氧化物酶标记的山羊抗兔 IgG 进行的间接免疫过氧化物酶技术表明，肿瘤小叶中央部分的纤连蛋白与肿瘤细胞密切相关。肿瘤组织内不存在纤维蛋白原和因子 VIII:Ag，表明纤连蛋白可能不是血浆或基质来源的，而免疫过氧化物酶数据表明纤连蛋白可能是 BCE 细胞的产物。
Nodular basal cell epitheliomas (BCE) contain fibro-nectin both within tumor nodules and at the nodulestroma interface (basement membrane zone). Fibronectin within or at the periphery of tumor nodules could be derived from the tumor cells, from entrapped stroma, or from plasma. The present study was designed to elucidate the source(s) of fibronectin within BCE nodules.If stromal entrapment occurred to any great extent, yon Willebrand factor VIII:Ag-stained blood vessels within tumor nodules should be evident by immunofluorescence techniques. Likewise, if plasma proteins were deposited in BCE, the tumor nodules should stain with fluorescein-conjugated antifibrinogen antibodies. Therefore, 6 basal cell epitheliomas were double labeled with rhodamine-conjugated antihuman fibronectin and fluorescein-conjugated antihuman factor VIII:Ag or fluorescein-labeled antihuman fibrinogen.Fibronectin was present in a linear disposition along the margin of tumor lobules and as a fine filamentous deposit in the central portions of tumor tissue. There was no evidence of fibrinogen or factor VIII:Ag in any of the tumor lobules. Factor VIII:Ag was present in a granular pattern within blood vessel walls that coursed between tumor nests. An indirect immunoperoxidase technique using rabbit antihuman fibronectin and peroxidase-labeled goat antirabbit IgG demonstrated that fibronectin within the central portion of the tumor lobules was closely associated with the tumor cells.The absence of fibrinogen and factor VIII:Ag within the tumor tissue indicates that the fibronectin is probably not plasma- or stroma-derived while immunoperoxidase data suggest that fibronectin may be a product of BCE cells.