Upregulation of TMEM16A Protein in Bronchial Epithelial Cells by Bacterial Pyocyanin.
Upregulation of TMEM16A Protein in Bronchial Epithelial Cells by Bacterial Pyocyanin.
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DOI：
10.1371/journal.pone.0131775
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Galietta LJ
中科院分区：
文献类型：
--
作者：
Caci E;Scudieri P;Di Carlo E;Morelli P;Bruno S;De Fino I;Bragonzi A;Gianotti A;Sondo E;Ferrera L;Palleschi A;Santambrogio L;Ravazzolo R;Galietta LJ
Th 2细胞因子诱导气道上皮粘液高分泌与TMEM 16 A（一种Ca 2+激活的Cl-通道）的表达相关。我们询问气道上皮细胞暴露于细菌成分（一种模拟囊性纤维化（CF）中发生的高度感染环境的条件）是否也会导致类似的反应。在培养的人支气管上皮细胞中，用绿脓菌素或用铜绿假单胞菌培养物上清液处理引起TMEM 16 A功能的显著增加。钙依赖性的Cl-分泌，触发的刺激与UTP，特别是增强绿脓菌素在CF患者的细胞。通过CF和非CF细胞中的免疫荧光证实了细菌组分引起的TMEM 16 A蛋白和MUC 5AC粘蛋白的表达增加。我们还通过免疫细胞化学研究了TMEM 16 A在人支气管中的表达。我们发现CF患者气道中的TMEM 16 A染色增加。CF粘膜下腺体信号最强。我们的研究结果表明，TMEM 16 A表达/功能在CF肺病中上调，可能是对气道中细菌存在的反应。
Induction of mucus hypersecretion in the airway epithelium by Th2 cytokines is associated with the expression of TMEM16A, a Ca2+-activated Cl- channel. We asked whether exposure of airway epithelial cells to bacterial components, a condition that mimics the highly infected environment occurring in cystic fibrosis (CF), also results in a similar response. In cultured human bronchial epithelial cells, treatment with pyocyanin or with a P. aeruginosa culture supernatant caused a significant increase in TMEM16A function. The Ca2+-dependent Cl- secretion, triggered by stimulation with UTP, was particularly enhanced by pyocyanin in cells from CF patients. Increased expression of TMEM16A protein and of MUC5AC mucin by bacterial components was demonstrated by immunofluorescence in CF and non-CF cells. We also investigated TMEM16A expression in human bronchi by immunocytochemistry. We found increased TMEM16A staining in the airways of CF patients. The strongest signal was observed in CF submucosal glands. Our results suggest that TMEM16A expression/function is upregulated in CF lung disease, possibly as a response towards the presence of bacteria in the airways.
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DOI：
10.1126/science.1255825
发表时间：
2014-08-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hoegger MJ;Fischer AJ;McMenimen JD;Ostedgaard LS;Tucker AJ;Awadalla MA;Moninger TO;Michalski AS;Hoffman EA;Zabner J;Stoltz DA;Welsh MJ
通讯作者：
Welsh MJ
影响因子：
24.3
作者：
Kohri, K;Ueki, IF;Nadel, JA
通讯作者：
Nadel, JA
影响因子：
4
作者：
Kim, Cheol Hong;Kim, Kyu-Earn;Song, Kyoung Seob
通讯作者：
Song, Kyoung Seob
影响因子：
15.9
作者：
Garcia, Mary Abigail S.;Yang, Ning;Quinton, Paul M.
通讯作者：
Quinton, Paul M.
DOI：
10.1152/ajplung.00311.2001
发表时间：
2002-02-01
影响因子：
4.9
作者：
Danahay, H;Atherton, H;Poll, CT
通讯作者：
Poll, CT