Assessment of neuronal maturation and acquisition of functional competence in the developing zebrafish olfactory system.
Assessment of neuronal maturation and acquisition of functional competence in the developing zebrafish olfactory system.
复制标题
评估发育中的斑马鱼嗅觉系统的神经元成熟和功能能力的获得。
DOI：
10.1023/b:mics.0000006852.16798.34
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Michel,WilliamC
中科院分区：
文献类型：
--
作者：
Sakata,Yoko;Olson,JaredK;Michel,WilliamC
嗅球水平的嗅觉编码被认为依赖于二尖瓣细胞的整体反应，二尖瓣细胞接受来自嗅觉感觉神经元的化学组织投射的输入，并受到侧抑制回路的调节。基于牛磺酸、谷氨酸、GABA（以及潜在的其他小生物胺）和胍基小阳离子（通过活跃的嗜离子性谷氨酸受体通道渗透来标记nmda敏感细胞）的相对浓度，描述了免疫细胞化学方法对发育中的斑马鱼嗅觉系统中的神经元进行代谢分类。利用代谢谱结合活动依赖标记，我们证明了发育中的嗅球的神经元分化，通过获得成熟的神经化学谱和对嗜离子性谷氨酸受体激动剂NMDA的敏感性进行评估，发生在发育的第二天。这种实验方法可能对有关谷氨酸信号通路发展的研究有用。
Olfactory coding at the level of the olfactory bulb is thought to depend upon an ensemble response of mitral cells receiving input from chemotopically-organized projections of olfactory sensory neurons and regulated by lateral inhibitory circuits. Immunocytochemical methods are described to metabolically classify neurons in the developing zebrafish olfactory system based on the relative concentrations of taurine, glutamate, GABA (and potentially other small biogenic amines) and a small guanidium-based cation, agmatine, which labels NMDA-sensitive cells by permeating through active ionotropic glutamate receptor channels. Using metabolic profiling in conjunction with activity dependent labeling we demonstrate that neuronal differentiation in the developing olfactory bulb, as assessed by acquisition of a mature neurochemical profile, and sensitivity to an ionotropic glutamate receptor agonist, NMDA, occurs during the second day of development. This experimental approach is likely to be useful in studies concerned with the development of glutamatergic signaling pathways.