CLONING AND EXPRESSION OF MURINE CD27 - COMPARISON WITH 4-1BB, ANOTHER LYMPHOCYTE-SPECIFIC MEMBER OF THE NERVE GROWTH-FACTOR RECEPTOR FAMILY
CLONING AND EXPRESSION OF MURINE CD27 - COMPARISON WITH 4-1BB, ANOTHER LYMPHOCYTE-SPECIFIC MEMBER OF THE NERVE GROWTH-FACTOR RECEPTOR FAMILY
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DOI：
10.1002/eji.1830230427
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发表时间：
1993-04-01
影响因子：
5.4
通讯作者：
LOENEN, WAM
中科院分区：
文献类型：
--
作者：
GRAVESTEIN, LA;BLOM, B;LOENEN, WAM
CD 27是神经生长因子受体家族的成员，该家族包括两种类型的肿瘤坏死因子受体，CD 40和Fas/Apo-1。人CD 27仅在淋巴细胞上发现。在T细胞中，其表达在抗原刺激后以瞬时方式强烈增加，表明CD 27在T细胞活化期间起作用。为了分析CD 27的功能，我们在cDNA和蛋白水平上鉴定了小鼠CD 27。与人CD 27相比，鼠CD 27显示出65%的同一性。氨基末端富含半胱氨酸的区域，i. e.推定的配体结合结构域和胞质结构域的羧基末端部分在人和小鼠中大约80%相同。小鼠CD 27与4-1BB具有29%的同一性，4 - 1BB是仅在cDNA水平上定义的受体家族的另一个淋巴细胞特异性成员。小鼠CD 27和4-1BB在富含半胱氨酸的结构域中具有39%的同源性，并且在胞质尾区中共享保守区域。表达研究在胸腺和脾脏中发现了小鼠CD 27 mRNA，但在非淋巴组织中未发现，而在任何检测组织中均未检测到4-1BB mRNA。在静息T细胞中，仅发现鼠CD 27 mRNA，而在活化T细胞中，鼠CD 27以及4-1BB以高水平存在。小鼠CD 27和4-1BB mRNA在T细胞活化过程中以不同的动力学表达，表明这些分子在该过程中发挥不同的作用。肽抗血清鉴定小鼠CD 27为胸腺细胞和活化T细胞上的45-kDa蛋白，而4-1BB从活化T细胞沉淀为35-40-kDa蛋白。
CD27 is a member of the nerve growth factor receptor family, that includes two types of tumor necrosis factor receptor, CD40 and Fas/Apo-1. Human CD27 has been found only on lymphocytes. In T cells, its expression strongly increases in a transient fashion upon antigenic stimulation, suggesting that CD27 plays a role during T cell activation. To analyze the function of CD27, we have identified the murine CD27 at the cDNA and protein level. Murine CD27 shows an identity of 65% compared with human CD27. The amino-terminal cysteine-rich region, i. e. the putative ligand-binding domain, and the carboxy-terminal part of the cytoplasmic domain are approximately 80% identical in man and mouse. Murine CD27 has 29% identity to 4-1BB, another lymphocyte-specific member of the receptor family defined only at the cDNA level. Murine CD27 and 4-1BB have 39% homology in the cysteine-rich domain and share a conserved region in the cytoplasmic tail. Expression studies identified murine CD27 mRNA in thymus and spleen, but not in non-lymphoid tissues, while 4-1BB mRNA was not detected in any tissue tested. In resting T cells, only murine CD27 mRNA was found, while in activated T cells murine CD27 as well as 4-1BB were present at high levels. Murine CD27 and 4-1BB mRNA are expressed with different kinetics during T cell activation, suggesting that these molecules play different roles in this process. Peptide antisera identified murine CD27 as a 45-kDa protein on thymocytes and activated T cells, while 4-1BB was precipitated as a 35-40-kDa protein from activated T cells.