Determination of Kinetic and Stoichiometric Parameters of Pseudomonas putida F1 by Chemostat and In Situ Pulse Respirometry
Determination of Kinetic and Stoichiometric Parameters of Pseudomonas putida F1 by Chemostat and In Situ Pulse Respirometry
复制标题
用恒化器和原位脉冲呼吸测定法测定恶臭假单胞菌 F1 的动力学和化学计量参数
DOI：
10.2202/1934-2659.1304
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发表时间：
2009
影响因子：
0.9
通讯作者：
F. Thalasso
中科院分区：
文献类型：
--
作者：
Catarina S. S. Oliveira;A. Ordaz;J. Alba;M. Alves;E. Ferreira;F. Thalasso
通过与传统的趋化方法的比较，评价了脉冲呼吸法在纯培养物动力学和化学计量参数估计中的适用性。以葡萄糖为唯一碳源，在连续搅拌式反应器中培养恶臭假单胞菌F1。反应器在0.06 ~ 0.35 h-1的6种稀释率下稳定运行。根据Monod模型，测量了底物浓度和生物量浓度，并用于估计动力学和化学计量参数。反应器采用原位呼吸测定法，注射葡萄糖脉冲19 ~ 97 mg L-1。根据ASM1和ASM3模型，用得到的呼吸图估计动力学和化学计量参数。趋化仪和呼吸法测定的参数无显著性差异。葡萄糖亲和常数为0.4 ~ 0.7 mg L-1，最大特定生长速率为0.14 ~ 0.20 h-1，生长产率为0.41 ~ 0.67。这些结果证实了原位脉冲呼吸法是一种适合于动力学和化学计量参数估计的方法。
The applicability of pulse respirometry, for the estimation of kinetic and stoichiometric parameters in pure cultures was evaluated by comparison with traditional chemostat method. Pseudomonas putida F1 was cultured in a continuous stirred tank reactor, using glucose as sole carbon source. The reactor was operated under steady-state with six dilution rates, ranging from 0.06 to 0.35 h-1. Substrate and biomass concentration were measured and used to estimate kinetic and stoichiometric parameters, according to the Monod model. An in situ respirometry method was also applied to the reactor, with the injection of pulses of glucose from 19 to 97 mg L-1. The respirograms obtained were used to estimate the kinetic and stoichiometric parameters according to ASM1 and ASM3 models. No significance difference was observed between parameters estimated by chemostat and respirometric methods. The glucose affinity constant was from 0.4 to 0.7 mg L-1, the maximum specific growth rate was from varying from 0.14 to 0.20 h-1, and the growth yield was from 0.41 to 0.67. These results confirm that in situ pulse respirometry is a suitable method for kinetic and stoichiometric parameters estimation.