Tunable lifetime multiplexing using luminescent nanocrystals
Tunable lifetime multiplexing using luminescent nanocrystals
复制标题
使用发光纳米晶体的可调寿命复用
DOI：
10.1038/nphoton.2013.322
复制
发表时间：
2014-01-01
期刊：
影响因子：
35
通讯作者：
Jin, Dayong
中科院分区：
文献类型：
--
作者：
Lu, Yiqing;Zhao, Jiangbo;Jin, Dayong
光学多路复用在诸如光学数据存储、文档安全、分子探针和用于个性化医学的珠粒测定等应用中起着重要作用。传统的荧光颜色编码受到光谱重叠和背景干扰的限制，限制了可区分身份的数量。在这里，我们表明，在微秒区域的可调发光寿命τ可以用来编码个别的上转换纳米晶体。在一个单一的色带中，可以产生十个以上的寿命从25.6 μs到662.4 μs不等的粒子群，并解码它们的寿命标识，这些标识与颜色和强度无关。这种“τ点”可能适合多通道生物成像、高通量细胞仪量化、高密度数据存储以及打击假冒的安全代码。该演示通过增加发光信号的时间维度扩展了光学多路复用能力，为生命科学，医学和数据安全开辟了新的机会。
Optical multiplexing plays an important role in applications such as optical data storage, document security, molecular probes,and bead assays for personalized medicine. Conventional fluorescent colour coding is limited by spectral overlap and background interference, restricting the number of distinguishable identities. Here, we show that tunable luminescent lifetimes τ in the microsecond region can be exploited to code individual upconversion nanocrystals. In a single colour band, one can generate more than ten nanocrystal populations with distinct lifetimes ranging from 25.6 µs to 662.4 µs and decode their well-separated lifetime identities, which are independent of both colour and intensity. Such ‘τ-dots’ potentially suit multichannel bioimaging, high-throughput cytometry quantification, high-density data storage, as well as security codes to combat counterfeiting. This demonstration extends the optical multiplexing capability by adding the temporal dimension of luminescent signals, opening new opportunities in the life sciences, medicine and data security.