Dynamic Changes of Vascular Endothelial Growth Factor and Angiopoietin-1 in Association With Circulating Endothelial Progenitor Cells After Severe Traumatic Brain Injury
Dynamic Changes of Vascular Endothelial Growth Factor and Angiopoietin-1 in Association With Circulating Endothelial Progenitor Cells After Severe Traumatic Brain Injury
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DOI：
10.1097/ta.0b013e31821ac9e1
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发表时间：
2011-06-01
影响因子：
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通讯作者：
Zhang, Jianning
中科院分区：
文献类型：
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作者：
Gong, Dasen;Zhang, Sai;Zhang, Jianning
背景：血管内皮生长因子（VEGF）和血管生成素-1（Ang-1）可促进脑损伤后血管新生和血管稳定性。循环内皮祖细胞（EPCs）在创伤性脑损伤（TBI）后的新生血管形成和组织修复中也起着至关重要的作用。我们试图比较VEGF和Ang-1的表达在血清和循环EPCs中的患者严重TBI后与健康controlsubjects.Methods：我们获得了外周血和血清样本从21例严重TBI和11名健康对照组。在严重TBI后1天、4天、7天、14天和21天通过流式细胞术定量来自严重TBI患者和健康对照的血液样品中的EPC。结果：与对照组相比，重型颅脑损伤组EPCs在伤后4d明显减少（p < 0.05），伤后7 d和14 d明显增加（p <0.05）; VEGF在随访期间显著升高（p < 0.05）。Ang-1逐渐升高，于伤后7 d和14 d达高峰。循环EPCs与VEGF和Ang-1在严重TBI后7天和14天显著相关。结论：我们的研究结果表明，VEGF和Ang-1的增加与严重TBI后循环EPCs的增加密切相关，这可能是严重TBI后血管修复所需的。
Background: Vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) can promote angiogenesis and vascular stability after brain injury. Circulating endothelial progenitor cells (EPCs) also play a crucial role in neovascularization and tissue repair after traumatic brain injury (TBI). We sought to compare the expression of VEGF and Ang-1 in serum and the circulating EPCs in patients after severe TBI with that of healthy control subjects.Methods: We obtained peripheral blood and serum samples from 21 patients with severe TBI and 11 healthy control subjects. EPCs in blood samples from severe TBI patients and healthy controls were quantified by flow cytometry 1 day, 4 days, 7 days, 14 days, and 21 days after severe TBI. VEGF and Ang-1 were measured by enzyme linked immunosorbent assay at the same time points.Results: Compared with control subjects, circulating EPCs in patients with severe TBI decreased 4 days (p < 0.05), but increased 7 days and 14 days (p < 0.05) after TBI. VEGF increased significantly during the follow-up period (p < 0.05). Ang-1 increased gradually and reached peak at 7 days and 14 days after TBI. The circulating EPCs were significantly correlated with VEGF and Ang-1 at 7 days and 14 days after severe TBI.Conclusions: Our results suggest that the increased VEGF and Ang-1 are closely related to increase in circulating EPCs in response to severe TBI, which may be needed for vascular repairs after severe TBI.