Antioxidant and pancreas-protective effect of aucubin on rats with streptozotocin-induced diabetes
Antioxidant and pancreas-protective effect of aucubin on rats with streptozotocin-induced diabetes
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DOI：
10.1016/j.ejphar.2007.12.011
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发表时间：
2008-03-17
影响因子：
5
通讯作者：
Xu, Yong-Ping
中科院分区：
文献类型：
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作者：
Jin, Lei;Xue, Hong-Yu;Xu, Yong-Ping
氧化应激被认为是糖尿病发生和并发症的一个重要因素。本研究旨在探讨桃红素对链脲佐菌素诱导的糖尿病大鼠胰腺脂质过氧化和抗氧化防御系统活性的保护作用，并对胰腺进行免疫组化评价。通过测定丙二醛浓度测定脂质过氧化，测定大鼠肝脏和肾脏中过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的活性。作为糖尿病病理的一部分，我们还研究了血糖变化和胰腺免疫组织化学评价。在我们的研究中，山竹素治疗降低了血糖。与对照组大鼠相比，糖尿病大鼠肝脏和肾脏的脂质过氧化水平升高，抗氧化酶活性降低。给糖尿病大鼠服用15天，可以显著逆转与糖尿病相关的损伤。此外，糖尿病大鼠胰腺胰岛素免疫反应性和β细胞数量明显下降，但紫苏桃素处理大鼠胰腺改善，免疫反应性β细胞数量明显增加。这些结果表明，桃红素可能作为一种安全的预防或治疗糖尿病的药物。(c) 2007 Elsevier B.V.版权所有
Oxidative stress has been suggested as a contributory factor in development and complication of diabetes. The aim of the present study was to determine the protective effect of aucubin on lipid peroxidation and activities of antioxidant defense systems and to conduct immunohistochemical evaluation of pancreas in streptozotocin-induced diabetic rats. Lipid peroxidation was determined by assessing the concentration of malondialdehyde and activities of antioxidant enzymes - catalase, glutathione peroxidase and superoxide dismutase in liver and kidneys of rats were determined. Changes of blood glucose and immunohistochemical evaluation on pancreas were also investigated as part of the pathology of diabetes. In our study, aucubin treatment lowered blood glucose. Diabetic rats exhibited an increase in the level of lipid peroxidation and decrease in activities of antioxidant enzymes in liver and kidneys as compared to control rats. Administration of aucubin to diabetic rats for 15 days significantly reversed damage associated with diabetes. In addition, diabetic rats showed an obvious decrease in insulin immunoreactivity and the number of beta cells in pancreas, but the pancreas of aucubin-treated rats were improved and the number of immunoreactive beta cells were significantly increased. These results indicated that aucubin may have value as a safe preventive or therapeutic agent against diabetes mellitus. (c) 2007 Elsevier B.V. All rights reserved.