Carbohydrate-Conjugated Amino Acid-Based Fluorescent Block Copolymers: Their Self-Assembly, pH Responsiveness, and/or Lectin Recognition
Carbohydrate-Conjugated Amino Acid-Based Fluorescent Block Copolymers: Their Self-Assembly, pH Responsiveness, and/or Lectin Recognition
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DOI：
10.1021/acs.langmuir.5b02245
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发表时间：
2015-09-01
期刊：
影响因子：
3.9
通讯作者：
De, Priyadarsi
中科院分区：
文献类型：
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作者：
Kumar, Sonu;Maiti, Binoy;De, Priyadarsi
通过可逆的加成断裂链转移(RAFT)聚合技术，将碳水化合物和氨基酸生物分子结合在一个单一的合成聚合体系中是一种有效的策略。这种独特的嵌段共聚物被设计成形成均匀的胶束，在外表面以多价方式选择性地或同时投影氨基酸/糖残基，提供基于pH的刺激响应性和/或凝集素识别。用场发射扫描电子显微镜(FE-SEM)、动态光散射(DLS)和紫外-可见光谱(UV-Vis)对自组装过程进行了详细研究。与仅含糖共聚物纳米粒相比，壳上同时具有氨基酸/糖实体的糖纳米粒的凝集素结合行为增强，这是因为在仅含糖共聚物纳米粒的情况下具有更高的空间位阻因子。利用荧光光谱进一步研究了后聚合功能化荧光团的偶联作用，证明了凝集素的识别过程。
An effective strategy has been documented to combine both carbohydrate and amino acid biomolecules in a single synthetic polymeric system, via a, reversible addition fragmentation chain transfer (RAFT) polymerization technique. The resultant unique block copolymer was engineered to form uniform micelles with the desired projection of either selective or both amino acid/sugar residues on the outer surface with multivalency, providing pH-based stimuli-responsiveness and/or lectin recognition. The self-assembly process was studied in detail by field emission scanning electron microscopy (FE-SEM), dynamic light scattering (DLS), and UV-visible spectroscopy. The enhanced lectin binding behavior was observed for glyconanoparticles with both amino acid/sugar entities on the shell as compared to the only glycopolymer nanoparticle because of the higher steric hindrance factor in the case of only the glycopolymer nanoparticle. Fluorophore conjugation by postpolymerization functionalization was further exploited by fluorescence spectroscopy for evidencing the lectin recognition process.