SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses
SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses
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SARS-CoV-2 蛋白质组微阵列，用于对 COVID-19 特异性 IgG 和 IgM 反应进行全局分析
DOI：
10.1038/s41467-020-17488-8
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发表时间：
2020-07-14
影响因子：
16.6
通讯作者：
Tao, Sheng-ce
中科院分区：
文献类型：
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作者：
Jiang, He-wei;Li, Yang;Tao, Sheng-ce
我们仍然对人类免疫系统如何对SARS-CoV-2做出反应知之甚少。在这里，我们构建了一个SARS-CoV-2蛋白质组芯片，其中包含28个预测蛋白质中的18个，并将其应用于29名康复患者血清中IgG和IgM抗体反应的表征。我们发现所有这些患者都有特异性结合SARS-CoV-2蛋白的IgG和IgM抗体，特别是N蛋白和S1蛋白。除了这些蛋白质，还鉴定了对ORF 9 b和NSP 5的显著抗体应答。我们发现，S1特异性IgG信号与年龄和乳酸脱氢酶（LDH）水平呈正相关，与淋巴细胞百分比呈负相关。总体而言，本研究提供了SARS-CoV-2特异性IgG和IgM反应的系统视图，并提供了帮助开发有效诊断，治疗和疫苗接种策略的见解。
We still know very little about how the human immune system responds to SARS-CoV-2. Here we construct a SARS-CoV-2 proteome microarray containing 18 out of the 28 predicted proteins and apply it to the characterization of the IgG and IgM antibodies responses in the sera from 29 convalescent patients. We find that all these patients had IgG and IgM antibodies that specifically bind SARS-CoV-2 proteins, particularly the N protein and S1 protein. Besides these proteins, significant antibody responses to ORF9b and NSP5 are also identified. We show that the S1 specific IgG signal positively correlates with age and the level of lactate dehydrogenase (LDH) and negatively correlates with lymphocyte percentage. Overall, this study presents a systemic view of the SARS-CoV-2 specific IgG and IgM responses and provides insights to aid the development of effective diagnostic, therapeutic and vaccination strategies.