Modulation of bronchial smooth muscle function in horses with heaves.
Modulation of bronchial smooth muscle function in horses with heaves.
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马匹气喘时支气管平滑肌功能的调节。
DOI：
10.1152/jappl.1994.77.5.2149
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Derksen,FJ
中科院分区：
文献类型：
--
作者：
Yu,MF;Wang,ZW;Robinson,NE;Derksen,FJ
研究了在正常马中调节气道平滑肌功能的四种机制，在气道阻塞性疾病引起的马的支气管中进行。将结果与同一实验室相同人员研究的历史对照数据进行比较。将左前叶支气管（LB 1）和小支气管（5 mm OD）的环悬挂在肌浴中，并测量等长张力。在LB 1中研究了抑制性非肾上腺素能非胆碱能（iNANC）功能。在用阿托品预处理LB 1节段并用组胺收缩后，电场刺激（EFS）诱导很少或没有舒张，表明在有起伏的马中iNANC功能障碍。哮喘动物的支气管对EFS和乙酰胆碱反应迟钝。上皮细胞去除增强收缩反应的小支气管乙酰胆碱的动物比对照组动物，表明上皮衍生的松弛因子的功能增强。相比之下，与对照马相比，用甲氨蝶呤（10（-6）M）抑制环氧合酶增加了受累马中EFS诱导的小支气管收缩，表明前列腺素产生的变化有利于兴奋性前列腺素类。我们的结论是，在马的支气管与起伏; iNANC功能是有缺陷的，平滑肌胆碱能激活的反应减少，上皮衍生的松弛因子的生产增强，前列腺素类的抑制功能减少。
Four mechanisms that modulate airway smooth muscle function in normal horses were studied in the bronchi of horses affected by the airway obstructive disease heaves. Results were compared with data from historical controls studied by the same personnel in the same laboratory. Rings from the left cranial lobar bronchus (LB1) and small bronchi (5 mm OD) were suspended in muscle baths, and the isometric tension were measured. The inhibitory nonadrenergic noncholinergic (iNANC) function was studied in LB1. After the LB1 segments were pretreated with atropine and contracted with histamine, electrical field stimulation (EFS) induced little or no relaxation, indicating iNANC dysfunction in horses with heaves. Bronchi from animals with heaves were hyporesponsive to EFS and acetylcholine. Epithelial removal augmented the contractile response of small bronchi to acetylcholine more in animals with heaves than in control animals, indicating an enhanced function of epithelial-derived relaxing factor. In contrast, cyclooxygenase inhibition with meclofenamate (10(-6) M) increased the EFS-induced contraction of small bronchi less in affected horses than in control horses, suggesting a change in prostaglandin production in favor of excitatory prostanoids. We conclude that in the bronchi of horses with heaves; the iNANC function is defective, the response of smooth muscle to cholinergic activation is diminished, the production of epithelial-derived relaxing factor is enhanced, and the inhibitory function of prostanoids is reduced.