IMMUNOHISTOCHEMICAL STUDIES ON TUMOR ANTIGEN-4 IN SQUAMOUS CELL CARCINOMA OF UTERINE CERVIX
IMMUNOHISTOCHEMICAL STUDIES ON TUMOR ANTIGEN-4 IN SQUAMOUS CELL CARCINOMA OF UTERINE CERVIX
复制标题
宫颈鳞状细胞癌肿瘤抗原4的免疫组织化学研究
DOI：
10.1267/ahc.20.75
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
I. Nishiya
中科院分区：
文献类型：
--
作者：
M. Matsuta;T. Kagabu;I. Nishiya
应用免疫组化方法对宫颈鳞状细胞癌（SCC）组织中肿瘤抗原4（TA-4）进行了定位研究。本文应用亲和素-生物素-过氧化物酶复合物法对92例浸润性宫颈癌石蜡包埋切片进行TA-4光镜检测。这些肿瘤在组织学上是SCC，包括68个大细胞非角化（LNK）型和24个角化（K）型。TA-4阳性细胞在LNK中的检出率为65%，在K中的检出率为100%。TA-4在正常鳞状上皮棘层细胞中也有定位，用包埋前间接免疫过氧化物酶法研究了TA-4的亚细胞定位。TA-4定位于肿瘤细胞的细胞质中，但不在张力丝中。提示TA-4是SCC的结构蛋白。因此，与角蛋白不同，TA-4可以被认为是SCC细胞分化的指标，TA-4应该是有用的肿瘤标志物，其表达鳞状成熟过程中的肿瘤组织的特征。
Immunohistochemical localization of Tumor Antigen 4 (TA-4) in squamous cell carcinoma (SCC) tissues of the uterine cervix was studied by means of the immunoperoxidase methods. Light microscopic detection of TA-4 was carried out by means of the avidin-biotin-peroxidase complex method on formalin-fixed paraffin-embedded sections from 92 cases of invasive cervical cancer of the uterus. These tumors were histologically SCC, consisting of 68 of the large cell non-keratinizing (LNK) type and 24 of the keratinizing (K) type. TA-4 positive cells were detected in 65% of LNK cases and 100% of K cases. Positively stained neoplastic cells were seen frequently around the hyperparakeratotic lesions, and they showed a tendency to keratinization.TA-4 localization was also confirmed in the cells of stratum spinosum of the non-cancerous squamous epithelium.Subcellular localization of TA-4 was studied by the preembedding indirect immunoperoxidase method. TA-4 was localized in the cytosols of the neo-plastic cells, but not in tonofilaments. It was suggested that TA-4 was a structural protein of SCC. Therefore, unlike keratin, TA-4 can be considered as an index of differentiation of SCC cells, and TA-4 should be useful as a tumor marker which expresses the characteristics of the neoplastic tissue during squamous maturation.