Induced pluripotent stem cells: applications in regenerative medicine, disease modeling, and drug discovery.
Induced pluripotent stem cells: applications in regenerative medicine, disease modeling, and drug discovery.
复制标题
DOI：
10.3389/fcell.2015.00002
复制
发表时间：
2015
影响因子：
5.5
通讯作者：
Chandra R
中科院分区：
文献类型：
--
作者：
Singh VK;Kalsan M;Kumar N;Saini A;Chandra R
诱导多能干细胞（Induced Pluripotent Stem Cells，iPSCs）领域的最新进展为治疗学研究开辟了许多途径。iPSC是使用不同转录因子从体细胞重编程的细胞。iPSC具有自我更新和分化为许多类型细胞谱系的独特性质。因此，它可以取代胚胎干细胞（ESC）的使用，并可能克服在研究和临床中使用胚胎的各种伦理问题。世界范围内，大量研究人员对iPSC的使用做出了压倒性的反应，这促使许多人建立了更可靠的iPSC生成方法。这就需要详细了解基本机制。已经有大量报道显示不同分子作为iPSC产生方法的推定调节剂的潜在作用。在从不同类型的体细胞来源重编程产生iPSC中发挥作用的分子机制涉及大量分子，包括miRNA，DNA修饰剂（即DNA甲基转移酶），NANOG等。有各种疾病已经通过使用iPSC进行建模，以更好地理解其病因，其可能进一步用于开发这些疾病的推定治疗。此外，iPSC用于生产患者特异性细胞，这些细胞可以移植到由于各种疾病状况而导致的损伤部位或组织变性部位。使用iPSC可以消除免疫排斥的机会，因为患者特异性细胞可以用于各种移植过程中的移植。此外，iPSC技术已用于各种疾病的疾病建模和基因治疗。该技术提供了优于其他类似技术，如动物模型的好处。人类和新设计的药物遇到的许多有毒化合物（不同的化合物、药物、其他危险化学品或环境条件）可以通过使用iPSC来评估毒性和影响。因此，iPSCs在再生医学、疾病建模和药物发现方面的应用是巨大的，应该以更全面的方式进行探索。
Recent progresses in the field of Induced Pluripotent Stem Cells (iPSCs) have opened up many gateways for the research in therapeutics. iPSCs are the cells which are reprogrammed from somatic cells using different transcription factors. iPSCs possess unique properties of self renewal and differentiation to many types of cell lineage. Hence could replace the use of embryonic stem cells (ESC), and may overcome the various ethical issues regarding the use of embryos in research and clinics. Overwhelming responses prompted worldwide by a large number of researchers about the use of iPSCs evoked a large number of peple to establish more authentic methods for iPSC generation. This would require understanding the underlying mechanism in a detailed manner. There have been a large number of reports showing potential role of different molecules as putative regulators of iPSC generating methods. The molecular mechanisms that play role in reprogramming to generate iPSCs from different types of somatic cell sources involves a plethora of molecules including miRNAs, DNA modifying agents (viz. DNA methyl transferases), NANOG, etc. While promising a number of important roles in various clinical/research studies, iPSCs could also be of great use in studying molecular mechanism of many diseases. There are various diseases that have been modeled by uing iPSCs for better understanding of their etiology which maybe further utilized for developing putative treatments for these diseases. In addition, iPSCs are used for the production of patient-specific cells which can be transplanted to the site of injury or the site of tissue degeneration due to various disease conditions. The use of iPSCs may eliminate the chances of immune rejection as patient specific cells may be used for transplantation in various engraftment processes. Moreover, iPSC technology has been employed in various diseases for disease modeling and gene therapy. The technique offers benefits over other similar techniques such as animal models. Many toxic compounds (different chemical compounds, pharmaceutical drugs, other hazardous chemicals, or environmental conditions) which are encountered by humans and newly designed drugs may be evaluated for toxicity and effects by using iPSCs. Thus, the applications of iPSCs in regenerative medicine, disease modeling, and drug discovery are enormous and should be explored in a more comprehensive manner.
登录
查看更多内容
影响因子：
64.5
作者：
Fong YW;Inouye C;Yamaguchi T;Cattoglio C;Grubisic I;Tjian R
通讯作者：
Tjian R
影响因子：
64.8
作者：
Batista, Luis F. Z.;Pech, MatthewF.;Zhong, Franklin L.;Nguyen, Ha Nam;Xie, Kathleen T.;Zaug, Arthur J.;Crary, Sharon M.;Choi, Jinkuk;Sebastiano, Vittorio;Cherry, Athena;Giri, Neelam;Wernig, Marius;Alter, Blanche P.;Cech, Thomas R.;Savage, Sharon A.;Pera, Renee A. Reijo;Artandi, Steven E.
通讯作者：
Artandi, Steven E.
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
21.3
作者：
Feng, Bo;Jiang, Jianming;Ng, Huck-Hui
通讯作者：
Ng, Huck-Hui
影响因子：
64.8
作者：
通讯作者：
--