Analyses of HBV cccDNA Quantification and Modification
Analyses of HBV cccDNA Quantification and Modification
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DOI：
10.1007/978-1-4939-6700-1_6
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Protzer, Ulrike
中科院分区：
文献类型：
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作者：
Xia, Yuchen;Stadler, Daniela;Protzer, Ulrike
共价闭合环状DNA （cccDNA）是乙型肝炎病毒（HBV）在感染细胞细胞核内复制的转录模板。即使复制被阻断，它也能确保HBV的持久性。免疫介导的被感染肝细胞的杀伤、细胞分裂或细胞因子诱导的cccDNA的非细胞溶解降解可诱导cccDNA的丢失。在HBV控制研究中，cccDNA的完整性分析及其精确定量是非常重要的。在这里，我们描述了HBV cccDNA定量和修饰的不同方法。
Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.