Transcriptomal comparison of human dermal lymphatic endothelial cells ex vivo and in vitro.
Transcriptomal comparison of human dermal lymphatic endothelial cells ex vivo and in vitro.
复制标题
人真皮淋巴内皮细胞离体和体外的转录比较。
DOI：
10.1152/physiolgenomics.00037.2006
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发表时间：
2007
影响因子：
4.6
通讯作者：
Kerjaschki,Dontscho
中科院分区：
文献类型：
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作者：
Wick,Nikolaus;Saharinen,Pipsa;Saharinen,Juha;Gurnhofer,Elisabeth;Steiner,CarlW;Raab,Ingrid;Stokic,Dejan;Giovanoli,Pietro;Buchsbaum,Sabine;Burchard,Aurea;Thurner,Stefan;Alitalo,Kari;Kerjaschki,Dontscho
淋巴内皮细胞在体内的功能依赖于其微环境，在体外不能完全复制。由于技术限制，未培养的“离体”淋巴内皮细胞中的基因表达尚未在分子水平上表征。采用组织微制备和细胞直接分离相结合的DNA芯片实验，在体外鉴定了159个分化人淋巴内皮细胞和血管内皮细胞的基因。对培养的原代细胞进行同样的分析表明，只有19个体外淋巴内皮特征基因在培养后保留了这种特性，而27个标记基因是新诱导的。此外，还可识别出一组泛内皮基因。淋巴内皮细胞在培养基中的繁殖刺激了与细胞更新、基本代谢和细胞骨架相关基因的转录。另一方面，编码细胞外基质组分、跨膜酪氨酸激酶信号通路和趋化因子（C-C）配体21的基因下调。淋巴内皮细胞转录组的直接离体分析有助于理解淋巴血管系统的生理学及其疾病的发病机制。
The in vivo functions of lymphatic endothelial cells depend on their microenvironment, which cannot be fully reproduced in vitro. Because of technical limitations, gene expression in uncultured, “ex vivo” lymphatic endothelial cells has not been characterized at the molecular level. We combined tissue micropreparation and direct cell isolation with DNA chip experiments to identify 159 genes differentiating human lymphatic endothelial cells from blood vascular endothelial cells ex vivo. The same analysis performed with cultured primary cells revealed that only 19 genes characteristic for lymphatic endothelium ex vivo retained this property upon culture, while 27 marker genes were newly induced. In addition, a set of panendothelial genes could be recognized. The propagation of lymphatic endothelial cells in culture stimulated transcription of genes associated with cell turnover, basic metabolism, and the cytoskeleton. On the other hand, there was downregulation of genes encoding extracellular matrix components, signaling via transmembrane tyrosine kinase pathways and the chemokine (C-C) ligand 21. Direct ex vivo analysis of the lymphatic endothelial cell transcriptome is helpful for the understanding of the physiology of the lymphatic vascular system and of the pathogenesis of its diseases.