Patterns of variation in alkamides and cichoric acid in roots and aboveground parts of Echinacea purpurea (L.) moench
Patterns of variation in alkamides and cichoric acid in roots and aboveground parts of Echinacea purpurea (L.) moench
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DOI：
10.21273/hortsci.40.5.1239
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发表时间：
2005-08-01
期刊：
影响因子：
1.9
通讯作者：
Davis, JM
中科院分区：
文献类型：
--
作者：
Qu, LP;Chen, Y;Davis, JM
我们研究了紫锥菊 (Echinacea purpurea (L.) Moench) 根部和地上部分中烷酰胺和菊苣酸积累的变化模式。这些植物化学物质是用 60% 乙醇从新鲜植物部分中提取的，并通过高效液相色谱 (HPLC) 分析进行定量。烷酰胺和菊苣酸的浓度以干重为基础测量（毫克中心点g(-1)）。对于总烷酰胺，根中的总烷酰胺浓度为 5.02 至 27.67（平均值 = 14.4%），近成熟种子头 (NMSH) 中的浓度为 0.62 至 3.42（平均值 = 1.54），年轻顶部（约 1/2 花头、1/4 叶和 1/4）中为 0.22 至 5.25（平均值 = 0.77）。茎）。对于菊苣酸，根部、NMSH 和顶部的菊苣酸浓度分别为 2.65 至 37.52（平均值 = 8.95）、2.03 至 31.58（平均值 = 10.9）和 4.79 至 38.55（平均值 = 18.88）。 Dodeca-2E、4E、8Z、10E-四烯酸异丁酰胺和 dodeca-2E、4E、8Z、10Z-四烯酸异丁酰胺（烷酰胺 8/9）仅占根中总烷酰胺的 9.5%，但在 NMSH 中占 87.9%，在嫩顶中占 76.6%。根和 NMSH 之间的烷酰胺或菊苣酸浓度的相关性不具有统计学显着性，无论是在根、NMSH 内还是在幼苗内。根中菊苣酸浓度与嫩枝中菊苣酸浓度呈显着负相关，根中总醇酰胺浓度与嫩枝中菊苣酸浓度呈显着正相关。这些结果可能有助于药用紫桉的遗传改良。
We investigated patterns of variation in alkamides and cichoric acid accumulation in the roots and aboveground parts of Echinacea purpurea (L.) Moench. These phytochemicals were extracted from fresh plant parts with 60% ethanol and quantified by high performance liquid chromatography (HPLC) analysis. Concentrations of alkamides and cichoric acid were measured on a dry-weight basis (mg center dot g(-1)). For total alkamides, concentrations among individual plants varied from 5.02 to 27.67 (mean = 14.4%) in roots, from 0.62 to 3.42 (mean = 1.54) in nearly matured seed heads (NMSH), and 0.22 to 5.25 (mean = 0.77) in young tops (about 1/2 flower heads, 1/4 leaves, and 1/4 stems). For cichoric acid, concentrations among individual plants varied from 2.65 to 37.52 (mean = 8.95), from 2.03 to 31.58 (mean = 10.9), and from 4.79 to 38.55 (mean = 18.88) in the roots, the NMSH, and the tops, respectively. Dodeca-2E, 4E, 8Z, 10E-tetraenoic acid isobutylamide and dodeca-2E, 4E, 8Z, 10Z-tetraenoic acid isobutylamide (alkamides 8/9) accounted for only 9.5% of the total alkamides in roots, but comprised 87.9% in the NMSH, and 76.6% in the young tops. Correlations of concentrations of alkamides or cichoric acid between those of roots and those of the NMSH were not statistically significant, and either within the roots, the NMSH, and the young tops. However, a significant negative correlation was observed between the concentration of cichoric acid in the roots and in young tops, and a significant positive correlation was observed between total alkamide concentration in the roots and cichoric acid concentration in the young tops. These results may be useful in the genetic improvement of E. purpurea for medicinal use.