Secretion of metalloproteinases by stimulated capillary endothelial cells. I. Production of procollagenase and prostromelysin exceeds expression of proteolytic activity.
Secretion of metalloproteinases by stimulated capillary endothelial cells. I. Production of procollagenase and prostromelysin exceeds expression of proteolytic activity.
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发表时间：
1986-02
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通讯作者：
G. S. Herron;Z. Werb;Dwyer Ks;M. J. Banda
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作者：
G. S. Herron;Z. Werb;Dwyer Ks;M. J. Banda
我们的特点是生物合成的两种金属蛋白酶，前胶原酶和prostromelysin，兔脑毛细血管内皮细胞（RBCE）通过免疫化学，生物合成，和功能测定。未受刺激的RBCE分泌没有检测到的金属蛋白酶。分泌的前胶原酶和prostromelysin诱导6小时内通过处理细胞与50 ng/ml的12-O-十四烷酰佛波醇-13-乙酸酯。在处理的细胞中，两种酶原占[35 S]蛋氨酸标记的分泌蛋白的20%; 10（6）RBCE在48小时内分泌约15微克每种蛋白。虽然RBCE分泌大约一样多的原胶原酶和prostromelysin的兔成纤维细胞，几乎没有酶活性可以测量RBCE条件培养基中，即使在激活的酶原胰蛋白酶或有机汞剂。
We have characterized the biosynthesis of two metalloproteinases, procollagenase and prostromelysin, by rabbit brain capillary endothelial cells (RBCE) by means of immunochemical, biosynthetic, and functional assays. Unstimulated RBCE secreted no detectable metalloproteinases. Secretion of both procollagenase and prostromelysin was induced within 6 h by treating the cells with 50 ng/ml 12-O-tetradecanoylphorbol-13-acetate. In treated cells, the two proenzymes accounted for up to 20% of the [35S]methionine-labeled secreted proteins; about 15 micrograms of each protein was secreted in 48 h by 10(6) RBCE. Although RBCE secreted approximately as much procollagenase and prostromelysin as did rabbit fibroblasts, virtually no enzyme activity could be measured in RBCE-conditioned medium, even after activation of the proenzymes by trypsin or an organomercurial agent.