An estrogen receptor pathway regulates the telogen-anagen hair follicle transition and influences epidermal cell proliferation
An estrogen receptor pathway regulates the telogen-anagen hair follicle transition and influences epidermal cell proliferation
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DOI：
10.1073/pnas.93.22.12525
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发表时间：
1996-10-29
影响因子：
11.1
通讯作者：
Smart, RC
中科院分区：
文献类型：
--
作者：
Oh, HS;Smart, RC
毛囊是一种循环的、自我更新的表皮结构，被认为是由来自真皮乳头的信号控制的，真皮乳头是真皮内的一群特化的间充质细胞。用17-β-雌二醇对小鼠的修剪的背部皮肤进行局部处理，使毛囊停滞在休止期，并在用生物学上无活性的立体异构体处理时产生对毛发生长的深刻和长期的抑制，用ICI 182，780（一种纯雌激素受体拮抗剂）局部处理引起毛囊退出休止期并进入生长期，从而启动毛发生长。皮肤中雌激素受体的免疫组织化学染色显示毛乳头细胞核的强烈和特异性染色，毛乳头中雌激素受体的表达具有毛发周期依赖性，其中休止期毛囊的表达水平最高，17-β-雌二醇处理的表皮表现出与对照表皮相似的5-溴-2 '-脱氧尿苷（BrdUrd）S期细胞数量;然而，对照表皮中BrdUrd S期基底细胞的数量根据下面毛囊的周期阶段而变化，范围从静止期毛囊以上的2.6%到早期生长期毛囊以上的7.0%，这些发现表明，毛乳头内的雌激素受体途径调节休止期，生长期毛囊转变，并表明与生长期毛囊相关的扩散因子影响表皮中的细胞增殖。
The hair follicle is a cyclic, self renewing epidermal structure which is thought to be controlled by signals from the dermal papilla, a specialized cluster of mesenchymal cells within the dermis, Topical treatments with 17-beta-estradiol to the clipped dorsal skin of mice arrested hair follicles in telogen and produced a profound and prolonged inhibition of hair growth while treatment with the biologically inactive stereoisomer, 17-alpha-estradiol, did not inhibit hair growth, Topical treatments with ICI 182,780, a pure estrogen receptor antagonist, caused the hair follicles to exit telogen and enter anagen, thereby initiating hair growth, Immunohistochemical staining for the estrogen receptor in skin revealed intense and specific staining of the nuclei of the cells of the dermal papilla, The expression of the estrogen receptor in the dermal papilla was hair cycle-dependent with the highest Levels of expression associated with the telogen follicle, 17-beta-Estradiol-treated epidermis demonstrated a similar number of 5-bromo-2'-deoxyuridine (BrdUrd) S-phase cells as the control epidermis above telogen follicles; however, the number of BrdUrd S-phase basal cells in the control epidermis varied according to the phase of the cycle of the underlying hair follicles and ranged from 2.6% above telogen follicles to 7.0% above early anagen follicles, These findings indicate an estrogen receptor pathway within the dermal papilla regulates the telogen-anagen follicle transition and suggest that diffusible factors associated with the anagen follicle influence cell proliferation in the epidermis.