Different Cold-Signaling Pathways Function in the Responses to Rapid and Gradual Decreases in Temperature
Different Cold-Signaling Pathways Function in the Responses to Rapid and Gradual Decreases in Temperature
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DOI：
10.1105/tpc.16.00669
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发表时间：
2017-04-01
期刊：
影响因子：
11.6
通讯作者：
Yamaguchi-Shinozaki, Kazuko
中科院分区：
文献类型：
--
作者：
Kidokoro, Satoshi;Yoneda, Koshi;Yamaguchi-Shinozaki, Kazuko
在植物中，寒冷的温度引发胁迫反应和长期反应，导致耐寒性。在拟南芥中，三个脱水反应元件（DRE）结合蛋白1/C-repeat结合因子（DREB 1/CBF）在冷响应基因表达中起着主开关的作用。DREB 1基因的诱导触发了冷胁迫诱导的转录级联反应，随后诱导了许多在冷胁迫反应和耐冷性中起作用的基因。许多参与DREB 1诱导的调节因子已经被确定，但这些因子如何协调DREB 1的冷胁迫特异性表达尚未阐明。在这里，我们揭示了植物识别冷胁迫作为两种不同的信号，快速和逐渐的温度下降，并诱导DREB 1基因的表达。钙调蛋白结合转运激活因子3（CAMTA 3）和CAMTA 5对温度的快速降低有反应，并诱导DREB 1的表达，但这些蛋白质对温度的逐渐降低没有反应。此外，它们在白天和晚上发挥作用，与一些关键的昼夜节律成分相反，包括昼夜节律时钟相关1和晚伸长下胚轴，它们仅在白天作为转录激活因子调节冷响应DREB 1表达。因此，植物有效地控制收购的抗冻性使用两种不同的信号通路，以响应在季节变化过程中的温度逐渐下降和夜间温度突然下降。
In plants, cold temperatures trigger stress responses and long-term responses that result in cold tolerance. In Arabidopsis thaliana, three dehydration-responsive element (DRE) binding protein 1/C-repeat binding factors (DREB1/CBFs) act as master switches in cold-responsive gene expression. Induction of DREB1 genes triggers the cold stress-inducible transcriptional cascade, followed by the induction of numerous genes that function in the cold stress response and cold tolerance. Many regulatory factors involved in DREB1 induction have been identified, but how these factors orchestrate the cold stress-specific expression of DREB1s has not yet been clarified. Here, we revealed that plants recognize cold stress as two different signals, rapid and gradual temperature decreases, and induce expression of the DREB1 genes. CALMODULIN BINDING TRANSCRIPTION ACTIVATOR3 (CAMTA3) and CAMTA5 respond to a rapid decrease in temperature and induce the expression of DREB1s, but these proteins do not respond to a gradual decrease in temperature. Moreover, they function during the day and night, in contrast to some key circadian components, including CIRCADIAN CLOCK ASSOCIATED1 and LATE ELONGATED HYPOCOTYL, which regulate cold-responsive DREB1 expression as transcriptional activators only during the day. Thus, plants efficiently control the acquisition of freezing tolerance using two different signaling pathways in response to a gradual temperature decrease during seasonal changes and a sudden temperature drop during the night.