Sustained release of vascular endothelial growth factor A and basic fibroblast growth factor from nanofiber membranes reduces oxygen/glucose deprivation-induced injury to neurovascular units.
Sustained release of vascular endothelial growth factor A and basic fibroblast growth factor from nanofiber membranes reduces oxygen/glucose deprivation-induced injury to neurovascular units.
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DOI：
10.4103/1673-5374.382252
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发表时间：
2024-04
影响因子：
6.1
通讯作者：
Hai J
中科院分区：
文献类型：
--
作者：
Wu Y;Sun J;Lin Q;Wang D;Hai J
上调脑缺血半暗带区血管内皮生长因子A/碱性成纤维细胞生长因子(VEGFA/bFGF)的表达，可增加血管体积，缩小病变体积，促进神经细胞增殖分化，从而发挥神经保护作用。然而，内源性VEGFA/bFGF的有益作用是有限的，因为它们的表达只是一过性的增加。在这项研究中，我们使用逐层自组装和静电纺丝技术制备了负载VEGFA/bFGF的多层纳米纤维膜。我们发现，含有10层的膜具有理想的超微结构，可以高效稳定地释放生长因子1个月以上。这种10层纳米纤维膜促进了脑微血管内皮细胞管的形成和增殖，抑制了神经元的凋亡，上调了紧密连接蛋白的表达，提高了神经血管单位各种细胞成分在缺氧/葡萄糖剥夺条件下的生存能力。此外，该纳米纤维膜还降低了Janus Kinase-2/信号转导和转录激活因子-3(JAK2/STAT3)、Bax/Bcl2的表达，并切割了caspase-3。因此，这种纳米纤维膜通过抑制JAK2/STAT3通路对缺氧/葡萄糖剥夺的神经血管单位表现出神经保护作用。
Upregulation of vascular endothelial growth factor A/basic fibroblast growth factor (VEGFA/bFGF) expression in the penumbra of cerebral ischemia can increase vascular volume, reduce lesion volume, and enhance neural cell proliferation and differentiation, thereby exerting neuroprotective effects. However, the beneficial effects of endogenous VEGFA/bFGF are limited as their expression is only transiently increased. In this study, we generated multilayered nanofiber membranes loaded with VEGFA/bFGF using layer-by-layer self-assembly and electrospinning techniques. We found that a membrane containing 10 layers had an ideal ultrastructure and could efficiently and stably release growth factors for more than 1 month. This 10-layered nanofiber membrane promoted brain microvascular endothelial cell tube formation and proliferation, inhibited neuronal apoptosis, upregulated the expression of tight junction proteins, and improved the viability of various cellular components of neurovascular units under conditions of oxygen/glucose deprivation. Furthermore, this nanofiber membrane decreased the expression of Janus kinase-2/signal transducer and activator of transcription-3 (JAK2/STAT3), Bax/Bcl-2, and cleaved caspase-3. Therefore, this nanofiber membrane exhibits a neuroprotective effect on oxygen/glucose-deprived neurovascular units by inhibiting the JAK2/STAT3 pathway.
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影响因子：
18.9
作者：
Jin L;Zhu Z;Hong L;Qian Z;Wang F;Mao Z
通讯作者：
Mao Z
影响因子：
5.6
作者：
Geiseler SJ;Morland C
通讯作者：
Morland C
DOI：
10.1093/brain/awu040
发表时间：
2014-05
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Barker R;Ashby EL;Wellington D;Barrow VM;Palmer JC;Kehoe PG;Esiri MM;Love S
通讯作者：
Love S
影响因子：
9.7
作者：
Huang, Zhixuan;Qian, Kun;Zhao, Liang
通讯作者：
Zhao, Liang
影响因子：
5.1
作者：
Guo, Sichao;Geng, Xiaokun;Ding, Yuchuan
通讯作者：
Ding, Yuchuan