VARIABLE PATTERNS OF TOTAL DNA AND RDNA METHYLATION IN ANIMALS
VARIABLE PATTERNS OF TOTAL DNA AND RDNA METHYLATION IN ANIMALS
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DOI：
10.1093/nar/8.7.1485
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
TAGGART, MH
中科院分区：
文献类型：
--
作者：
BIRD, AP;TAGGART, MH
限制性内切酶被用来确定广泛的动物DNA中OCGG和GCGC序列的甲基化程度。对总DNA甲基化和核糖体DNA甲基化进行了研究。整个DNA甲基化在节肢动物中可检测到，在其他无脊椎动物门中有部分甲基化，在脊椎动物中高度甲基化。核糖体DNA在所有动物中主要是非甲基化的，除了鱼类和两栖动物，在这些动物中核糖体DNA高度甲基化。我们讨论了这些结果的进化和功能含义，并认为基因组类型之间的巨大差异是高度甲基化和甲基化的间隔的相对大小进化变化的结果。
Restriction endonucleases were used to determine the degree of methylation at the sequences OCGG and GCGC in a wide range of animal DNAs. Both total DNA methylation and ribosomal DNA methylation were studied. Whole DNA methylation was indetectable in arthropods, fractional in other invertebrate phyla, and high in the vertebrates. Ribosomal DNA was predominantly unmethylated in all animals except fish and amphibia, where it was heavily methylated. We discuss the evolutionary and functional implications of these results, and suggest that the large differences between genome types are the result of evolutionary changes in the relative size of heavily methylated and urmethylated compartments.