Proliferative capacity of murine hematopoietic stem cells in vitro.
Proliferative capacity of murine hematopoietic stem cells in vitro.
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小鼠造血干细胞的体外增殖能力。
DOI：
10.1126/science.7071580
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Hellman,S
中科院分区：
文献类型：
--
作者：
Reincke,U;Hannon,EC;Rosenblatt,M;Hellman,S
从小鼠骨髓细胞长期培养的上清液中可以反复收集大量的粒细胞。粘附的造血干细胞的数量和每个培养物的寿命细胞产量之间存在恒定的关系。这些数据表明，正常干细胞和辐照干细胞的增殖能力是有限的。在从以有限稀释液置于基质培养层中的“米色”C57（bg/bgJ）干细胞的克隆培养物产生标记的粒细胞中发现了类似的限制。
Large numbers of granulocytes can be collected repeatedly from the supernatant medium of long-term cultures of mouse bone marrow cells. A constant relationship was found between the number of adherent hematopoietic stem cells and the lifetime cell production per culture. The data indicate that there is a limit to the proliferative capacity of normal and of irradiated stem cells. A similar limitation was found in the production of marked granulocytes from clonal cultures of "beige" C57 (bg/bgJ) stem cells placed in limiting dilutions into stromal culture layers.