FlyGEM, a full transcriptome array platform for the Drosophila community -: art. no. R19

FlyGEM, a full transcriptome array platform for the Drosophila community -: art. no. R19
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DOI:
10.1186/gb-2004-5-3-r19
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发表时间:
2004-01-01
期刊:
影响因子:
12.3
通讯作者:
Eastman, S
Eastman, S
中科院分区:
生物学1区
文献类型:
--
作者:
Johnston, R;Wang, B;Eastman, S

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我们构建了 DNA 微阵列来监测果蝇中预测基因的表达。通过使用同型杂交,我们表明该阵列具有可重复性,染料效应最小,并且阵列结果与系统性 Northern 印迹一致。阵列基因列表已被广泛注释并链接到其他数据库。 Incyte 和 NIH 通过 NIH 委员会选择的学术微阵列设施向社区提供了该平台。
We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.