Cell Heterogeneity Revealed by On-Chip Angiogenic Endothelial Cell Migration
Cell Heterogeneity Revealed by On-Chip Angiogenic Endothelial Cell Migration
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DOI：
10.1021/acsomega.9b03074
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发表时间：
2020-02
期刊：
影响因子：
4.1
通讯作者：
Tianze Xie;Nan Li;Sifeng Mao;Qiang Zhang;Jin‐Ming Lin
中科院分区：
文献类型：
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作者：
Tianze Xie;Nan Li;Sifeng Mao;Qiang Zhang;Jin‐Ming Lin
血管新生是肿瘤组织发育和内渗的关键过程，血管芽的生长是由单个内皮细胞（ECs）的迁移决定的。本文提出了一种用微通道模拟容器芽的芯片上分析方法来研究单个ec的迁移。当存在化学刺激时，观察到ec会单独向因子源迁移，而不是集体迁移。通过胶质瘤细胞共培养和掺杂血管内皮生长因子（VEGF） 165的培养基诱导EC迁移，证明了该方法的有效性。细胞位移与VEGF 165浓度呈正相关。通过对单个细胞的跟踪，可以反映细胞间迁移能力的差异，从而揭示细胞对刺激敏感性的异质性。
In sprouting angiogenesis, a key process involved in the development and the intravasation of tumor tissues, the growth of vessel sprouts, is determined by migration of single endothelial cells (ECs). This paper presents an on-chip assaying method to investigate the migration of individual ECs by simulating vessel sprouts with microchannels. When chemical stimulus is present, ECs were observed to migrate individually toward the source of factors instead of migrating collectively. The validity of this method is shown by inducing EC migration with glioma cell coculture and culture media doped with vascular endothelial growth factor (VEGF) 165. A positive correlation between cell displacement and VEGF 165 concentration was observed. Difference in migrating ability among cells was reflected by tracking single cells, which could reveal cell heterogeneity in susceptibility to stimulus.