CHARACTERIZATION OF THE ADIPOKINETIC HORMONE-RECEPTOR FROM THE FAT-BODY OF MANDUCA-SEXTA
CHARACTERIZATION OF THE ADIPOKINETIC HORMONE-RECEPTOR FROM THE FAT-BODY OF MANDUCA-SEXTA
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DOI：
10.1016/0167-0115(95)00046-e
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发表时间：
1995-06-27
影响因子：
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通讯作者：
MORIMOTO, H
中科院分区：
文献类型：
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作者：
ZIEGLER, R;JASENSKY, RD;MORIMOTO, H
合成了一种氚标记的天南星脂肪动力激素（M-AKH）（pE-L-T-[p（3）H]F-T-S-S-W-G-NH_2）（比活度27 Ci/mmol），其在生物测定中是完全活性的。将其用于基于过滤的结合测定以表征来自M.六。从脂肪体制备膜组分，并确定最佳结合条件。K-d为7。10（-10）M，受体浓度估计为0.5 pmol/mg膜蛋白。用M.脑、心脏和右肌制备的膜均未发现受体结合。sexta或来自蟑螂Blaberus discoidalis的脂肪体。然而，特异性结合被发现与膜制剂从翼胸神经节的M。六。来自神经节的膜具有比脂肪体膜少得多的结合位点，然而，结合是特异性的，并且在每个实验中观察到。
A tritium labeled Manduca sexta adipokinetic hormone (M-AKH) was synthesized (pE-L-T-[p(3)H]F-T-S-S-W-G-NH2) (specific activity 27 Ci/mmol) which was fully active in a bioassay. It was used in a filtration based binding assay to characterize the M-AKH receptor from the fat body of M. sexta. Membrane fractions were prepared from fat body and optimal binding conditions were determined. A K-d of 7 . 10(-10) M was determined and the receptor concentration estimated to be 0.5 pmol/mg membrane protein. No receptor binding was found when membranes were prepared from brain, heart or Right muscle of M. sexta or from fat body of the cockroach Blaberus discoidalis. However, specific binding was found with membrane preparations from the pterothoracic ganglion of M. sexta. The membranes from the ganglion had a much smaller number of binding sites than the fat body membranes, however, the binding was specific and observed in each experiment.