Difference in chilling-induced flavonoid profiles, antioxidant activity and chilling tolerance between soybean near-isogenic lines for the pubescence color gene
Difference in chilling-induced flavonoid profiles, antioxidant activity and chilling tolerance between soybean near-isogenic lines for the pubescence color gene
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DOI：
10.1007/s10265-010-0345-2
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Hajika, Makita
中科院分区：
文献类型：
--
作者：
Toda, Kyoko;Takahashi, Ryoji;Hajika, Makita
耐冷性是大豆的一项重要性状。[MERR.]产自凉爽的气候。我们以前分离到了一个与控制毛发和种皮颜色的T基因座相对应的大豆类黄酮3‘羟基酶(F3’H)基因。T基因和耐冷性之间的遗传联系已有报道，尽管确切的潜在机制尚不清楚。以大豆近等基因系To7B(TT)和To7G(TT)为材料，研究了冷害、抗氧化活性与低温处理(15A℃)相关的类黄酮谱之间的关系。To7G次三叶冷害比To7B严重。在7G低温下，过氧化氢积累和脂质过氧化作用增强。在低温诱导下，To7B的抗氧化活性比To7G更显著。高效液相色谱分析表明，冷藏处理后，To7B次生三叶叶片中3‘，4’-二羟基黄酮衍生物的含量增加，山奈酚糖苷(4‘-单羟基黄酮醇衍生物)含量增加。组织化学染色也显示了低温诱导的类黄酮类积聚。基因芯片分析和实时定量逆转录-聚合酶链式反应表明，大豆F3‘H基因的转录水平在低温条件下上调。这两个近等基因系在冷害、抗氧化活性和类黄酮种类上的差异支持这样的观点，即大豆F3‘H通过产生3’，4‘-二羟基黄酮醇衍生物来提高抗氧化活性，从而影响耐冷性。
Chilling tolerance is an important trait of soybeans [Glycine max (L.) Merr.] produced in cool climates. We previously isolated a soybean flavonoid 3' hydroxylase (F3'H) gene corresponding to the T locus, which controls pubescence and seed coat color. A genetic link between the T gene and chilling tolerance has been reported, although the exact underlying mechanisms remain unclear. Using the soybean near-isogenic lines (NILs) To7B (TT) and To7G (tt), we examined the relationship between chilling injury, antioxidant activity and flavonoid profiles associated with chilling treatment (15A degrees C). Chilling injury was more severe in the second trifoliate leaves of To7G than in those of To7B. Hydrogen peroxide accumulation and lipid peroxidation were enhanced by chilling in To7G. Chilling-induced enhancement of antioxidant activity was more prominent in To7B than in To7G. High performance liquid chromatography analysis indicated that the contents of quercetin glycosides and isorhamnetin glycosides (3',4'-dihydroxylated flavonol derivatives) increase in the second trifoliate leaves of To7B after chilling treatment, whereas the same treatment increased kaempferol glycoside (4'-monohydroxylated flavonol derivatives) content in the corresponding leaves of To7G. Histochemical staining also demonstrated chilling-induced flavonoid accumulation. Microarray analysis and real-time reverse transcription-PCR demonstrated that the transcript levels of soybean F3'H are upregulated by chilling. The differences in chilling injury, antioxidant activity and flavonoid species between the two NILs support the notion that soybean F3'H affects chilling tolerance by increasing antioxidant activity via production of 3',4'-dihydroxylated flavonol derivatives.