A simple inexpensive method for the production of tissue microarrays from needle biopsy specimens - Examples with prostate cancer
A simple inexpensive method for the production of tissue microarrays from needle biopsy specimens - Examples with prostate cancer
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DOI：
10.1097/00129039-200503000-00016
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发表时间：
2005-03-01
影响因子：
1.6
通讯作者：
Kajdacsy-Balla, A
中科院分区：
文献类型：
--
作者：
Datta, MW;Kahler, A;Kajdacsy-Balla, A
组织微阵列的使用已成为一种有效的方法，用于高通量分析的组织与分子标记，但这些研究还没有被用来利用有限的材料中存在的针活检的人体组织。这些活检组织的使用对于研究那些通过非手术方法治疗的患者的疾病至关重要，这些方法包括放疗、化疗或姑息治疗。作者提出了一种简单，廉价的方法，使用针吸活检标本的组织微阵列。使用这个过程与前列腺癌标本，作者证明，超过150个载玻片可以从一个单一的区域的癌症在针吸活检和核心的长度所涉及的癌症在针吸活检决定了可用的组织微阵列载玻片的数量。作者还指出，从单个患者活检中需要最佳数量的样本（3个），以确保有足够的材料进行分析，并在针吸活检和手术切除组织微阵列样本之间进行定量标记Ki-67的免疫组织化学相关性。这个过程可以扩展到任何类型的穿刺活检标本，增加研究的数量和这些组织作为实际应用的潜在用途。
The use of tissue microarrays has become an efficient method for the high-throughput analysis of tissues with molecular markers, yet these studies have not been used to leverage the limited materials present in needle biopsies of human tissues. The use of these biopsy tissues is crucial to study diseases in patients who are treated by nonsurgical methods such as radiation, chemotherapy, or palliative care. The authors present a simple, inexpensive method for using needle biopsy specimens in tissue microarrays. Using this process with prostate cancer specimens, the authors demonstrate that over 150 slides can be produced from a single area of cancer in a needle biopsy and that the length of the core involved by cancer in the needle biopsy determines the number of available tissue microarray slides. The authors also note the optimal number of samples (three) needed from a single patient biopsy to guarantee sufficient material for analysis and perform an immunohistochemical correlation between needle biopsy and surgical resection tissue microarray samples for the quantitative marker Ki-67. This process can be extended to any type of needle biopsy specimen, increasing the number of studies and potential use of these tissues as a practical reality.